Comparing the Effect of Galbanic Acid and Chitosan Gel on Pro-Inflammatory Factors in Intra-Abdominal Adhesions after Laparotomy in Rats
Subject Areas : Journal of Animal BiologyFiroozeh Saghaei 1 , melika montazeri 2 , Mahsa Rezvan 3
1 - Department of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Department of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 - Department of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Keywords: Laparotomy, Intra-abdominal adhesions, Inflammatory cytokines, Galbanic acid, Chitosan.,
Abstract :
Abdominal adhesions are a common complication after laparotomy (abdominal surgery) that can cause intestinal obstruction, pelvic pain and infertility. Since inflammation plays an effective role in induction of intra-abdominal adhesions and galbanic acid has anti-inflammatory and anti-cancer effects, in the present research, its effect on inhibiting of pro-inflammatory factors such as TNF-α and IL-1β, in abdominal adhesions after laparotomy was investigated. For this purpose, after anesthetizing and opening the abdomen, adhesion was induced in male wistar rats and, adhesion was induced in male wistar rats, and in one group, 2 cc of chitosan gel was injected in the adhesion induction site and the abdomen was closed, and in one group, galbanic acid (10mg/ kg) was injected intraperitoneally for 7 days. At the end of 7 days, the abdomen was opened again and the abdominal adhesion was observed and a sample was taken from the adhesion sites. RT-PCR method was used to determine the gene expression of pre-inflammatory factors TNF-α and IL-1β. The results of the study showed that intra-peritoneal injection of galbanic acid like chitosan gel effectively reduced the incidence of intra-abdominal adhesions after laparotomy. This effect was accompanied by a decrease in TNF-α and IL-1β gene expression in both groups, which indicates the anti-inflammatory effect of these two natural substances.
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