The Effectiveness of 8 Weeks of Aerobic Training on CT and TT Genotypes of rs2070744 Polymorphism of NOS3 Gene and Changes in Aerobic Performance of Untrained Women
Subject Areas : Journal of Animal Biologyhadis rahimi 1 , mania roozbayani 2 , abbas saremi 3
1 - Department of Physical Education, Borujerd Branch, Islamic Azad University, Borujerd, Iran
2 - Department of Physical Education, Borujerd Branch, Islamic Azad University, Borujerd, Iran
3 - Department of Physical Education, Arak Branch, Islamic Azad University, Arak, Iran
Keywords: CT and TT genotypes, NOS3, Aerobic exercise, Obesity, VO2max,
Abstract :
The present study was conducted with the aim of investigating the effect of 8 weeks of aerobic training on the CT and TT genotypes of the rs2070744 polymorphism of the NOS3 gene and the changes in aerobic performance of untrained women. For this purpose, 29 inactive women aged 30 to 45 were randomly selected from volunteer women in Shahriar city. The subjects did 8 weeks of aerobic training with a frequency of 5 sessions per week and each session lasting 30 minutes with an intensity of 55 to 75% of the reserve heart rate, so that in the first two weeks with 55 to 65% of the maximum heart rate, in two weeks Second, they trained with 60 to 65% of the maximum heart rate and the last 4 weeks with 65 to 75% of the maximum heart rate. 10 minutes for warming up and 10 minutes for cooling down were considered in each training session. Bruce's 7-step test was used to determine VO2max before and after training.Then, among the subjects who were able to perform the desired test based on the researcher's expectation, saliva sampling was done for DNA sequencing to determine the genotypes. The RFLP method was used to determine the genotype. The results of the data analysis were analyzed using the dependent t-test and the results showed that the VO2max level in women with CT genotype was not significant before and after the exercise intervention (p = 0.015), the amount of VO2max in women who had the TT genotype was not significant before and after the exercise intervention (p = 0.110). In this research, it was shown that the significant improvement of Vo2max did not depend on their genotypic differences, and no significant relationship was observed between the CT and TT genotypes of the rs2070744 polymorphism of the NOS3 gene and the changes in aerobic performance of obese untrained women after 8 weeks of aerobic training.
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