Evaluating the effect of Morphine and its combination with Naloxone, Cannabinoid receptor type 1 inhibitor (Am251), phospholipase inhibitor (U73122), and Pregabalin on Viability of YM1 Esophageal Cancer Cells
Subject Areas : Journal of Animal Biology
Hossain Pourkargar
1
(Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran )
mahyar ansari
2
(Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran )
Keywords: Esophageal cancer cell line YM1, Morphine, naloxone, Am251, U73122, Pregabalin,
Abstract :
It has been found that morphine may affect proliferation of cancer cells in addition to the effect of analgesia. In this study, the effects of morphine, naloxone, cannabinoid receptor type 1 inhibitor (Am251), phospholipase C inhibitor (U73122) and pregabalin were evaluated on YM1 esophageal cancer cell line viability. Esophageal cancer cell line YM1 cells were propagated in RPMI-1640 medium containing 10% FBS. Then the cells were treated with different doses of drugs for 24 hours and the cell viability was determined by the MTT method. The results were analyzed by one-way ANOVA and LSD post hoc test. The investigation showed that morphine in all three doses (87, 175 and 350 μM) significantly reduced the viability of cells (p < 0.05). The addition of 60 μM naloxone to the culture medium could not change the survival rate of esophageal cancer cells. But naloxone along with 350 μM morphine significantly decreased the survival rate compared to the control group (p < 0.001) and the naloxone group (p < 0.02). Phospholipase C inhibitor (U23177, 1mM) alone (P < 0.001) or together with 350 µM morphine decreased cell viability (p < 0.001). Also, AM251 140 μM alone (p < 0.05) or together with morphine decreased cell viability (p < 0.001). Pergabalin with a concentration of 6 μM with 6 mM alone (p < 0.05) or together with morphine decreased cell viability compared to the control group (p < 0.001) and the ratio of the 350 μM morphine group (p < 0.01). Also, cell viability in the group that was exposed to all three compounds was greatly reduced (p < 0.001). The results showed that morphine and the drugs investigated in this study can be used as potential options to reduce cell growth in patients with advanced esophageal cancer.
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