Evaluation of miR-191 Expression Level in Peripheral Blood Cells of Women with Breast Cancer Compared with Healthy Women

Subject Areas : Journal of Animal Biology

Arezou Shahi ¹ , Naghmeh Bahrami ² , Robab Rafii Tabatabaii ³ , Abodolreza Mohammadinia ⁴

1 - Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 - Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
4 - 4- Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, IranMedical Sciences, Tehran, Iran.

Received: 2020-10-22 Accepted : 2021-04-14 Published : 2021-05-22

Keywords: breast cancer, biomarker, miR-191,

Abstract :

Breast cancer is the most common cancer in women. Micro RNAs have emerged as a biomarker for the diagnosis and treatment of breast cancer. This study aimed to evaluate miR-191 in the peripheral blood of patients with breast cancer. 80 peripheral blood samples (40 patients with breast cancer and 40 healthy individuals) were collected. Immediately, RNA extraction and cDNA synthesis were performed, and polymerase chain reaction (Real-time RT-PCR) was performed. Dedicated primer design: Dedicated primers were designed and manufactured by the manufacturer using AlleleID6 software. The data were analyzed using paired t-test. The data obtained from the present study showed that the blood level of miR-191 was significantly higher in the patients with breast cancer compared to the healthy individuals. The evaluation of the relative expression level of the Mir-191 gene in patients' peripheral blood cells can be used as a biomarker to detect breast cancer in women.

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