Protective Effect of Vitamin C on Liver Tissue and Function in Female Newborns from the Mothers Treated with Lead Acetate
Subject Areas : Journal of Animal Biology
مرضیه
سلطانیان
1
(گروه زیست شناسی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران)
مهرداد
شریعتی
2
(گروه زیست شناسی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران)
اسفندیار
شریفی
3
(گروه زیست شناسی، واحد کازرون، دانشگاه آزاد اسلامی، کازرون، ایران)
Keywords: liver, vitamin C, Lead acetate,
Abstract :
Vitamin C is a simple compound of 6 carbons and it can be found in vegetable foods, and it has important roles in the body. Lead is one of the heavy metals and basic elements of igneous rocks of earth crust. Lead is produced from lead filtration but the machines is taking into account from important resources lead production in cities. This material is absorbed by respiratory system, digestive system, and skin. This study due to the importance of liver role in the body and having high rates of lead in environment, by the aim of reviewing protection effect of Vitamin C on the activity of liver enzyme, such as Alanine Transaminase (ALT), Aspartate Transaminase (AST), Alkaline Phosphate (ALP), and the some biomechanical factors of blood like protein and albumin is done. The rats which fed and curry with acetate is done. In this study, 48 male rats (Wistar strain) from the mothers which classified and fed with specified doses, and found in experiment groups 3 and 4, at first 100 mg/kg lead acetate and to the order 500, 250 mg/kg vitamin C was found in pregnant period, and were divided into 6 group of 8th. Control group did not administrated by any drugs and sham group was found distilled water (as a solvent). The drugs is given to the mothers in all period of pregnant as edible. After ending the pregnancy period and in 22th days, the blood sampling was done. The time of test is accounted 42 days (control groups, sham, experiment 1,2,21 days and experiment group 3,4,42 days.).The evaluation of level rate of enzyme plasma ALP, ALT, ASP, albumin, protein was done diagnosis kits of Pars Azmoon Company by RA-1000 machine. Also, histology review was done by staining Hematoxylin – Eosin. The results which received from the tests were analyzed by statistical analysis of Tukey Tests, ANOVA Test and T-Test.The comparison of statistical test results from larvae weight is indicated that experiment groups which received different rates of drug rather than control group showed significant reduction in the level of P≤ 0.05, and among experiment which received different dose of drug and control group, there was seen significant increase in liver enzyme, ALT, ALP, AST... In a manner that in maximum receiver of drug was seen significant increasing rather than control group. Also, the results show that the changes in liver weight and the rate of albumin and protein serum in experiment groups of drug receiver from control group is not significant. In tissue samples produced by increasing rate of drug, was seen more necrosis.
