The Effect of Synchronus Intraperitoneal Injection of Harmaline and PTZ on Serotonergic and Gabaergic Pathways and Seizure Threshold in NMRI Male Mice
Subject Areas : Journal of Animal Biologyغلامحسن واعظی 1 , فاطمه نصیرزاده 2
1 - دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران
2 - دانشگاه آزاد اسلامی، واحد دامغان، گروه زیست شناسی، دامغان، ایران
Keywords: Mice, PTZ, Harmaline, Seizure Threshold, Serotonergic pathway, Gabaergic pathway,
Abstract :
Epilepsy is one of the chronic disorders of nervous system in human being. Totally, this disease is distinguished by motor, sensory and psychic periodic attacks. This disease disrupts the brain function and it is accompanied by different, abrupt, transitory and returnable symptoms that usually, the conscious level gets disruptive. The goal of this research is the study of intraperitoneal injection effects of harmaline and PTZ on seizure threshold and its effect on serotonergic and gabaergic pathways. The experiments were carried out on male mice (NMRI) with approximateweigh of 25-30 gr. In this study, the animals divided into five groups of eight: experimental and sham. Sham groups received intraperitoneal injection of harmaline (dose of 5mg/kg) and intraperitoneal injection of PTZ (dose of 45mg/kg). Three experimental groups received synchronous injection of PTZ (dose of 45mg/kg) and different doses of harmaline (5, 10, 20 mg/kg). After drug injection, seizure reflexes have been recorded through direct observation for 20 minutes. The obtained information was analyzed by applying statistical tests (Tukey and ANOVA). The results of this study have shown that in sham group seizure threshold of PTZ was a dose of 45mg/kg and harmaline seizure thresholdwas a dose of 5mg/kg. Whereas, in pre-test applying of low doses has not shown any symptoms of tremor and seizure, also harmaline in case of dependent dose and significantly has reduced seizures threshold. The results of this study have shown that synchronous and intraperitoneal injection of harmaline and PTZ have reduced the seizure threshold and seizure symptoms have revealed earlier.
