Characterization, biological evaluation, and investigation of antibacterial properties of strontium-modified silicate-based bioactive glass in the presence of high amounts of magnesium
Subject Areas :
Niloufar Kolivand
1
(
Department of Materials Engineering, Imam Khomeini International University, Qazvin 34149-16818, Iran
)
Amirhossein Moghanian
2
(
Department of Materials Engineering, Imam Khomeini International University, Qazvin 34149-16818, Iran
)
Fathollah Moztarzadeh
3
(
Biomaterial Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, Iran
)
Majid Raz
4
(
Department of Engineering, Shahryar branch, Islamic azad university, Shahryar, Iran
)
Keywords: Silicate-based bioactive glass, Hydroxyapatite, In vitro, Strontium, Magnesium,
Abstract :
In this research, silicate-based bioactive glasses modified with strontium oxide based on 60SiO2-(36-x)CaO-4P2O5-5SrO-(8,10)MgO (molar percentage) were synthesized by sol-gel method and the bioactive properties their in vitro properties were investigated by characterization tests, biological evaluations and antibacterial tests against Mrsa bacteria (MRSA). According to the results of X-ray fluorescence spectroscopy (XRF), the amount of change in the amount of elements on average before and after synthesis, for SBG8M and SBG10M samples was equal to 6.202±4.08% and 4.99±2.66%, respectively, which indicates a negligible effect the synthesis process is based on the final chemical composition of the samples. Also, according to the X-ray diffraction (XRD) results, the characteristic peaks of hydroxyapatite (HA) were observed only in the SBG8M sample after 14 days of immersion in the simulated body solution (SBF), which Scanning electron microscopy (SEM) was also confirmed. Besides, according to the results of biological evaluations, increasing the amount of magnesium oxide from 8 to 10 mol percent, led to a decrease of 6.74%, 4.87%, and 17.65% in the amount of optical density (OD) and also a decrease of 9.93%, 12.16% and 24.30% in alkaline phosphatase (ALP) activity on days 1, 3 and 7 respectively of culture in vitro culture medium. In addition, increasing the amount of magnesium oxide led to a decrease of 18.53% in antibacterial properties. Therefore, the presence of high amounts of 8 and 10 mol percent magnesium oxide in the chemical composition of bioactive glasses leads to a decrease in their in vitro bioactivity and antibacterial properties.
