A Cytometric Analysis in Circulating Blood Lymphocytes of Water pipe Tobacco Smokers and Non-Smokers by Cytokinesis-block Micronucleus Technique: A Genomic Health Study on Apparent Healthy Premenopausal Women in Tehran

Subject Areas : Journal of Chemical Health Risks

Negar Bahrami ¹ , Sepideh Arbabi Bidgoli ² , Ramin Abrashami ³ , Aziz Mahmoudzadeh ⁴ , Masoumeh Heshmati ⁵

1 - Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 - Pharmaceutical Sciences Branch, Islamic azad University
3 - Department of Clinical Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4 - Faculty of Passive Defense, Malek Ashtar University of Technology, Iran
5 - Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Received: 2022-12-31 Accepted : 2024-05-06 Published : 2024-05-06

Keywords: Waterpipe smoking, MNi frequency, Genomic health, Tehran,

Abstract :

According to the association between increased micronuclei (MNi) level in reproductive age women and increased risk of congenital abnormalities, we aimed in this study to find the contribution of WTS to women's health in apparently healthy young cases by comparing the variability of MN values in water pipe smokers and non-smokers. Finding the associations between MNi levels and demographic, socioeconomic, reproductive, and lifestyle factors was the secondary scope of this study. This cross-sectional case-control study was performed on 30 premenopausal women (15 cases and 15 controls) who lived in Tehran, according to our inclusion criteria. The mean MN frequency among waterpipe smokers was 28.53± 7.462, whereas the same parameter in the control group was 6.53 + 3.24 (p= 0.001). Non-parametric tests revealed a significant association between MNi and frequency of waterpipe smoking (p = 0.001), age of starting waterpipe smoking (p = 0.003), concurrent use of alcoholic drinks (p= 0.004), and secondhand smoking (p =0.001). In the context of heavy environmental pollution in Tehran, significantly higher MNi frequencies and decreased genomic health in waterpipe smoker women in reproductive age may predispose them to an increased risk of harmful reproductive outcomes. These findings emerge from governmental and non-governmental biomonitoring programs in high-risk women to concern more on unhealthy lifestyles and environmental pollution.

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