Comparison inducible candidate gene expression patterns under salinity stress in bread wheat (Triticum aestivum L.)
Subject Areas : New Finding in AgricultureM. Gomarian 1 , M. A. Malbooi 2 , F. Darvish 3 , S. A. Mohammadi 4
1 - Department of plant breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Associated professor, National Institute for Genetic Engineering and Biotechnology, Plant Biotechnology Group, Tehran, Iran
3 - Department of plant breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Full Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Keywords: Gene expression, Wheat, Susceptibility, Tolerance, long term salt stress, transcription,
Abstract :
In order to investigation intra specious variation to salinity tolerance in wheat gene expression patterns were compared in salt tolerant and sensitive wheat under control and stress conditions. Reverse northern blot technique was used to compare gene expression patterns. To simplify, sixteen gene expression patterns were considered in salt tolerant and sensitive genotype in control and stress conditions. The gene expression patterns of the studied genes were located in ten out of sixteen gene expression patterns. The most important expression patterns were number 2, 6, 8 and 12. More genes were placed in the expression pattern of the number 2 than to other expression patterns. The majority of the transcription factor expressions were located in the four above gene expression patterns. Seven genes up regulated in both sensitive and tolerant genotypes in the present of salt stress. In the current study, four genes were selected as long term salt tolerant candidate genes in Mahooti cultivar including LEA, CBEFP, bZIP5 and wsr3 genes from second, sixth, eighth and twelfth expression patterns, respectively. The results also indicated that a larger number of salt responsive transcripts were expressed in tolerant genotype. These results show that differences in the gene expression patterns in varieties within species may produce salt stress tolerant and sensitive genotypes.
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