Agronomic and physiological response of Nigella sativa L. to Ascorbate and methanol foliar application in water deficit stress

Subject Areas : Journal of Plant Ecophysiology

Mojtaba Baradaran Firouzabadi ¹ , Mahdie Parsaeian ² , Mehdi Baradaran Firouzabadi ³

1 - M.Sc. in plant medicine, Islamic Azad University, Karaj
2 - Assistant professor in plant breeding, Department of agronomy, Shahrood University of Thechnology
3 - Associate professor in agronomy, Department of agronomy, Shahrood University of Thechnology

Received: 2015-07-30 Accepted : 2017-07-22 Published : 2017-11-22

Keywords: Chlorophyll, dry matter, Oil yield, Nigella sativa L,

Abstract :

Nowadays, the application of antioxidant and osmotic regulator substances is discussed in order to reduce the negative effects of various stresses. Ascorbate and methanol as the one of these substances cause resistant to stresses in plant. In order to examine this subject in plant medicinal (Nigella sativa L.), the split plot factorial experiment based on randomized complete block design was conducted in three replications in University of Shahrood at 2011. The main factor was irrigation levels (8 and 16 day interval as no stress and severe stress) and sub factors were foliar application of methanol (0, 15 and 30 %V) and ascorbate (0, 10 and 20 mM). Foliar applications of treatments were applied 45 and 55 days after planting. The results indicated that, dry matter accumulation in leaf and stem, 1000 seed weight, number of secondary branches in plant, relative water content and membrane stability index were decreased by water deficit stress significantly. Foliar application of ascorbate and methanol especially in the highest concentration increased all traits except 1000 seed weight in stress and no-stress conditions. The higher amount of relative water content and membrane stability index were obtained from highest concentrations of ascorbate and methanol together. Also, oil yield increased 27.8 percentages in this treatment. Therefore, in this experiment combination of 20 Mm ascorbate and 30 %V methanol is advisable.

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