Determination of pathotype diversities of Puccinia triticina Erikson (causal agent of wheat brown rust) in Iran based on rDNA ITS1 sequencing

Subject Areas : Mycology

Ali Reza Niazmand ¹ ( Department of Plant Pathology, Jahrom Branch, Islamic Azad University, Jahrom, Iran )
Shahab Haj Mansoor ² ( Department of Plant Pathology, Research and Science Branch, Islamic Azad University, Tehran, Iran )

Received: 2013-04-14 Accepted : 2013-04-14 Published : 2010-10-01

Keywords: Wheat, Sequencing, ITS1, Brown rust,

Abstract :

Background and Objective: Wheat brown rust is one of the most important diseases caused by Puccinia triticina Erikson. In recent years, determination of Brown rust pathotypes based upon rDNA ITS1 sequencing has become more interested. The aim of this research was evaluation the similarities and differences ITS1 of ribosomal DNA between the brown rust pathotypes collected from different geographical and climatically parts of Iran. Material and Methods: The DNA extraction was performed on urediniospore of collected sample. The ITS5 and Rust 2 primers were used for PCR amplification of rDNA ITS1 region, and the amplified products were sequenced directly. The accession numbers of isolate sequences were registered in GeneBank. Multiple sequence alignments and identification of tandem repeats were carried out by MPEG4 and DNA MAN softwares. Results: The results showed %0-1.2 differences between ITS1 sequences. Different mutations, includingdeletion, insertion, number of tandem repeat and some differences in ITS1 length were observed in DNA sequences. . Conclusion: Despite differences in pathotype, phenotype and geographical distributions of isolates, only limited differences were observed in ITS1 sequences. Because no logical correlation was observed between differences in pathotypes and ITS1 sequences, this rDNA region cannot be used as marker for determination of pathotypes of these fungi.  

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