Determination of pathotype diversities of Puccinia triticina Erikson (causal agent of wheat brown rust) in Iran based on rDNA ITS1 sequencing

Subject Areas : Mycology

Ali Reza Niazmand ¹ , Shahab Haj Mansoor ²

1 - Department of Plant Pathology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
2 - Department of Plant Pathology, Research and Science Branch, Islamic Azad University, Tehran, Iran

Received: 2013-04-14 Accepted : 2013-04-14 Published : 2010-10-01

Keywords: Wheat, Sequencing, ITS1, Brown rust,

Abstract :

Background and Objective: Wheat brown rust is one of the most important diseases caused by Puccinia triticina Erikson. In recent years, determination of Brown rust pathotypes based upon rDNA ITS1 sequencing has become more interested. The aim of this research was evaluation the similarities and differences ITS1 of ribosomal DNA between the brown rust pathotypes collected from different geographical and climatically parts of Iran. Material and Methods: The DNA extraction was performed on urediniospore of collected sample. The ITS5 and Rust 2 primers were used for PCR amplification of rDNA ITS1 region, and the amplified products were sequenced directly. The accession numbers of isolate sequences were registered in GeneBank. Multiple sequence alignments and identification of tandem repeats were carried out by MPEG4 and DNA MAN softwares. Results: The results showed %0-1.2 differences between ITS1 sequences. Different mutations, includingdeletion, insertion, number of tandem repeat and some differences in ITS1 length were observed in DNA sequences. . Conclusion: Despite differences in pathotype, phenotype and geographical distributions of isolates, only limited differences were observed in ITS1 sequences. Because no logical correlation was observed between differences in pathotypes and ITS1 sequences, this rDNA region cannot be used as marker for determination of pathotypes of these fungi.  

References:

1. Abbasy M, Ershad J, Hajarood G. Taxonomy of Puccinia recondita s. lat. causing brown rust on grasses in Iran. Iran J Plant Pathol. 2005; 4(41): 9-15. [In Persian].

2. Kolmer JA. Tracking wheat rust on a continental scale. Curr Opin Plant Biol. 2005; 8: 441-449.

3. Marasas CN, Smale M, Singh RP. The economic impact in developing countries of leaf rust resistance breeding in CIMMYT related spring bread wheat. Mexico, DF: International Maize and Wheat Improvement Center. 2004; 38P.

4. Roelfs AP, Singh RP, Saari EE. Rust diseases of wheat: concepts and methods of disease management. Mexico, DF: CIMMYT. 1992; 81P.

5. Chester KS. The nature and prevention of the cereal rusts as exemplified in the leaf rust of wheat. Waltham, MA: Chronica Botanica. 1946; 269P.

6. Abdel Hak TM, EL- Sherif NA, Bassiouny A, Shafik I A, EL Dauadi Y. Control of wheat leaf rust by systemic fungicides. Proceedings of the Fifth European and Mediberranean Cereal Rusts Conference. Bari, Italy, 1980; 255-266.

7. Hanzalova A, Huszar J, Bartos P, Herzova E. Occurrence of wheat leaf rust  Puccinia triticina  races and virulence changes in Slovakia in 1994-2004. J Biol. 2008; 63 (2): 171-174.

8. Kassem M, El-Ahmed A, Shafik HM, Nachit F. Identification of physiological races of Puccinia triticina Eriks, Wheat leaf rust in Northern Syria and South Turkey.  Ninth Arab Congress of Plant Protection; 2006 19-23 November 2006; Damascus, Syria. 2006; 109.

9. Long DL, Kolmer JA, Leonard KJ, Hughes ME. Physiologic specialization of Puccinia triticina on wheat in the United States in 2000. Plant Dis. 2002; 86(9): 981-986.

10. Lind V, Gultyaeva E. Virulence frequences of Puccinia triticina in Germany and the European regions of the Russian Federation. J Phytopathol. 2007; 155(1):13-21.

11. Bamdadian A. Physiologic races of Puccinia recondita in Iran (1968-1972). Cereal Rust Bull. 1973; 1: 45-47.

12. Torabi M, Nazari K, Afshari F. Genetics of pathogenicity of Puccinia recondita f.sp. tritici, the causal agent of leaf rust of wheat. Iran J Agr Sci. 2001; 32: 635.

13. Afshari F, Torabi M, Kia S, Dadrezai T, Safavi SA, Chaichi M, et al. Monitoring of virulence factors of Puccinia triticina, the causal agent of wheat leaf rust in Iran during 2002-2004. Seed and Plant Improvement Journal. 2006; 21(4): 485-501. [In Persian].

14. Niazmand AR, Afshari F, Abbasi M, Rezaee S. Study on pathotype diversity and virulence factors of puccinia triticina Erikson, the causal agent of wheat brown rust in Iran. Iran J Plant Pathol. 2010; 46(3): 187-202. [In Persian].

15. McDonald BA, Martinez JP. DNA restriction fragment lenght polymorphism among Mycosphaerella graminicola (anamorph Septoria tritici) isolates collected from a single field. Phytopathology. 1990; 80(12): 1368-73.

16. Henson JM, French R. The polymerase chain reaction and plant disease diagnosis. Annu Rev Phytopathol. 1993; 31: 81-109.

17. Egger KN. Molecular analysis of ectomycorrhizal fungal communities. Can J Bot. 1995; 73(1): 1415-1422.

18. Ennos RA, McConnell KC. Using genetic markers to measure natural selection in populations of fungi. Can J Bot. 1995; 73: 302-310.

19. Bridge PD, Arora DK. Applications of PCR in Mycology. In: Bridge PD, Arora DK, Reddy CA, Elander RP (eds). Interpretation of PCR methods for species definition: CAB International. 1998; pp. 63-84.

20. Takamatsu S, Hirata T, Sato Y. Phylogenetic analysis and predicted secondary structures of the rDNA internal transcribed spacers of the powdery mildew fungi (Erysiphaceae). Mycoscience. 1998; 39(4): 441-53.

21. Nazar RN, Hu X, Schmidt J, Culham D, Robb J. Potential use of PCR-amplified ribosomal intergenic sequences in the detection and differentiation of Verticillium wilt pathogens. Physiol Mol Plant Path. 1991; 39(1): 1-11.

22. Zambino PJ, Szabo LJ. Phylogenetic relationships of selected cereal and grass rusts based on rDNA sequence analysis. Mycologia. 1993; 85(3): 401-414.

23. Jennings JM, Newton AC, Buck KW. Detection of polymorphism in Puccinia hordei using RFLP and RAPD markers, differential cultivars and analysis of the intergenic spacer region of rDNA. J Phytopathol. 1997; 145(11-12): 511-519.

24. Kim WK, Zerucha T, Klassen GR. A region of heterogeneity adjacent to the 5s ribosomal RNA gene of cereal rusts. Curr Genet. 1992; 22(2): 101-105.

25. Abbasi M, Goodwin SB, Scholler M. Taxonomy, phylogeny, and distribution of Puccinia graminis, the black stem rust: new insights based on rDNA sequence data. Mycoscience. 2005; 46(4): 241-247.

26. Reader U, Broda P. Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol. 1985; 1(1): 17-20.

27. Hillis DM, Dixon MT. Ribosomal DNA: molecular evolution and phylogenetic inference. Q Rev Biol. 1991; 66(4): 411-453.

28. Driessen S, O'Brien PA, Hardy GESJ. Diversity of Puccinia boroniae assessed by teliospore morphology and restriction fragment patterns of ribosomal DNA. Australas Plant Pathol. 2004; 33 (1): 77-82.

29. Alaei H, De Backer M, Nuytinck J, Maes M, Höfte M, Heungens K. Phylogenetic relationships of Puccinia horiana and other rust pathogens of Chrysanthemum x morifolium based on rDNA ITS sequence analysis. Mycol Res. 2009; 113 (6-7): 668-683.

30. Barnes CW, Szabo LJ. Detection and identification of four common rust pathogens of cereals and grasses using realtime polymerase chain reaction. Phytopathology. 2007; 97(6): 717-727.