Isolation of Staphylococcus simulans, a lysostaphin-producing organism, from bovine mastitis and its identification based on 16S rRNA sequencing method

Subject Areas : Applied Microbiology

Sajjad Yazdan Setad ¹ , Majid Moghbeli ² , Hatef Ajoudanifar ³

1 - کارشناس ارشد، دانشگاه آزاد اسلامی، واحد دامغان، دانشکده علوم زیستی، گروه میکروبیولوژی
2 - استادیار، دانشگاه آزاد اسلامی، واحد دامغان، دانشکده علوم زیستی، گروه میکروبیولوژی
3 - استادیار، دانشگاه آزاد اسلامی، واحد دامغان، دانشکده علوم زیستی، گروه میکروبیولوژی

Received: 2012-10-21 Accepted : 2013-01-19 Published : 2013-04-01

Keywords: 16S rRNA, Staphylococcus simulans, Lysostaphin, Bovine mastitis,

Abstract :

Background and Objectives:  S. simulans is a gram-positive cocci that can be isolated  from skin and urine, as well as some clinical samples in human and animals, such as bovine mastitis. The present study was aimed to isolate and identify S. simulans, a lysostaphin-producing organism, from bovine mastitis and to test therapeutic property of this species against the staphylococcal infections. Materials and Methods: This cross- sectional study was done on the collected samples from breast of the cows afflicted to mastitis in Damghan, Semnan Province. The samples were cultured on nutrient agar and were examined based on Gram staining and biochemical tests. Then, the genomic DNA of the samples suspected for S. simulans were extracted. The accurate identification of the bacteria and tracking of lysostaphin gene were evaluated by using PCR and sequencing. Results: One out of 61 gram-positive isolated cocci from bovine mastitis samples was detected as S. simulans. This result was verified using 16S rRNA sequencing.  Conclusion: This study is the first report of isolation of S. simulans, a lysostsphin-producing organism, from bovine mastitis. This species is potentially useful for extraction of lysostsphin which is applied for treatment of the infections caused by antibiotic resistance bacteria.

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