The survey of frequency Chlamydiae  in semen used for artificial insemination

Subject Areas : Bacteriology

Ali  Sharifzadeh ¹ (Department of Microbiology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran)
mohsen  forooghi ² (. Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahre kord, Iran)

Received: 2021-03-15 Accepted : 2022-08-13 Published : 2021-08-23

Keywords: PCR, insemination, artificial, Chlamydiae,

Abstract :

Background & Objectives: Chlamydia spp. is an obligate intracellular agent that causes           chlamydiosis in animals and humans. Chlamydia infections in cows can cause abortion, infertility and other symptoms. The aim of this survey was to investigate the frequency of chlamydia        infection in sperm used by artificial insemination.Materials & Methods: In This survey semen samples were collected from semen supply centers. Then DNA was extracted using Cinnagen company kit. The samples were Analysed for the     chlamydial agent by Nested polymerase chain reaction (Nested PCR) by 16SrRNA gene primer and then the PCR product was electrophoresed and positive samples were identified by genus and species.Results: The finding of this survey indicated the prevalence of infection of different chlamydial species in the sperm samples. A Total of 100 samples were tested, 23 samples were positive for chlamydia spp.which are 12 positive samples separately for Chlamydia Pecorum,Chlamydia   abortus 7 positive samples and 4 positive samples were identified for Chlamydia Psitassi.Conclusion: The present survey showed that sperm samples can be an important source of      chlamydial infection transmission in bulls.