Lubrication and oil recovery by biosurfactant produced by Acinetobacter johnsonii ABR6

Subject Areas : Microbial Biotechnology

Elham  Akbari ¹ (Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran)
Kaivan  Beheshti Maal ² (Associate Professor in Microbial Biotechnology, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.)
Behnam  Rasekh ³ (Microbiology and Biotechnology Research Group , Research Institute of Petroleum Industry , Tehran , Iran)
Zarrin dokht  Emami ⁴ (Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran)
Meisam  Omidi ⁵ (Medical Nanotechnology & Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran)

Received: 2020-04-08 Accepted : 2020-08-16 Published : 2020-08-22

Keywords: Biosurfactant, Acinetobacter johnsonii, Crude oil lubrication,

Abstract :

Background and Objectives: Solid and semi-solid wastes are produced as oil sludge at different stages of crude oil refining. Accumulation of oil waste in the refinery reduces the efficiency of oil refining and its release causes environmental pollution.  The purpose of this study is to lubricate crude oil in pipelines and recycle oil using a biosurfactant produced by an indigenous strain.  Materials and Methods: The biosurfactant-producing isolates were obtained from petroleum reservoir in Isfahan Oil Refinery, Isfahan, Iran. Screening was performed by oil displacement method. Also the surface tension was measured by tensiometer. Biosurfactant chemical structure was identified by using chemical analysis. Oil recovery from the sludge was measured under controlled condition. The effect of biosurfactant lubrication was investigated on crude oil in the pipelines in vitro. The stability of biosurfactant in different environmental conditions was also determined. Results: The best biosurfactant-producing bacterium was identified as Acinetobacter johnsonii ABR6, and its 16S-rDNA genomic sequence was deposited in GenBank under the accession number of MK100470. Chemical analysis of TLC and FTIR confirmed that the produced biosurfactant was lipopeptide. The biosurfactant obtained from this bacterium recovered 50% of crude oil from petroleum sludge and also reduced transportation speed from 64 to 35 seconds. This biosurfactant had high stability in 5% w/v NaCl, pH range of 8 to 10, temperature of 60°C and autoclave conditions.  Conclusion: The results of this study showed that the biosurfactant produced by the native strain of Acinetobacter Johnson ABR6, in addition to operating in extreme conditions, also has the ability to recover oil and increase the transfer rate of crude oil in pipelines. Therefore, the use of this biosurfactant can be considered as an asset in the petroleum industry

References:

1. Singh A, Singh B, Ward O. Potential applications of bioprocess technology in petroleum
   industry. Biodeg. 2012; 23,865–880.
   2. Araújo S, Silva-Portela B, Lima, DC. A biosurfactant protein derived from a metagenomic
   library with activity in oil degradation. Sci Rep. 2020; 10, 1340.
   3. Bendaha M, Mebrek S, Naimi M. Isolation and comparison of rhamnolipids production in
   Pseudomonas aeruginosa P.B:2 and Pseudomonas fluorescens P. . Inter Biodet
   Biodeg,2012; 1–7444.
   4. Banat IM and et al. Cost effective technologies and renewable substrates for biosurfactants'
   production. fmicb, 2014; 5 697. 12
   5. Banat IM, Franzetti A, Gandolfi I, Bestetti G and et al. Microbial biosurfactants production,
   applications and future potential. Appl Microb Biotech.2010; 87:427-444.
   6. Fenibo E, Ijimo G, Selvarjan R, Chiekere C, Microbial Surfactants: The Next Generation
   Multifunctional Biomolecules for Applications in the Petroleum Industry and Its Associated
   Environmental Remediation. Microb. 2019; 7(11): 581.
   7. Ghorbannezhad H, Moghimi H, Dastgheib M. Biodegradation of high-molecular-weight
   aliphatic and aromatic hydrocarbons by Aspergillus calidoustus, J of Micro.World.2018. 10:
   4,346-359. [In Persian]
   8. Alvarez M, Jurelevicius D, Marques JM, Souza PM and et al. Bacillus amyloliquefaciens
   TSBSO 3.8, a biosurfactant-producing strain with biotechnological potential for microbial
   enhanced oil recovery. Coll Surf B Biointerf.2015; 136:14-21.
   9. EI-Sheshtawy HS, Doheim M Selection of Pseudomonas aeruginosa for biosurfactant
   production and studies of its antimicrobial activity. Egyp J Petr.2014; 23:1-6..
   10. El-Sheshtawy HS, Aiad I, Osman ME, Abo-ELnasr AA, Kobisy AS. Production of
   biosurfactant from Bacillus licheniformis for microbial enhanced oil recovery and inhibition
   the growth of sulfate reducing bacteria. Egyp J Petr.2015; 24:155–162.
   11. Gudiña EJ, Fernandes EC, Rodrigues AI, Teixeira JA and et al. Biosurfactant production by
   Bacillus subtilis using corn steep liquor as culture medium. Front Microb. 2015; 59:1-7..
   12. He C, Dong W, Li J, Li Y, Huang C, Ma Y. Characterization of rhamnolipid biosurfactants
   produced by recombinant Pseudomonas aeruginosa strain DAB with removal of crude oil.
   Biotech Lett.2017; 39:1381-1388.
   13. Akbari E, Beheshti-Maal K, Nayeri H. Production and optimization of alkaline lipase by a
   novel psychrotolerant and halotolerant strain Planomicrobium okeanokoites ABN-IAUF-2
   isolated from Persian Gulf. Inter Jl Med Res & Heal Scie.2016; 5:139–148.
   14. Akbari E, Beheshti-Maal K, Nayeri H. A novel halo-alkalo-tolerant bacterium, Marinobacter
   alkaliphilus ABN-IAUF-1, isolated from Persian Gulf suitable for alkaline lipase production.
   IJEST.2018; 15:1767-1776.
   15. Jafari A, Raheb J, Bardania H and et al. Isolation, cloning and expression of rhamnolipid
   operon from Pseudomonas aeruginosa ATCC 9027 in logarithmic phase in E. coli BL21. Ame
   J lif Scie.2015; 2:22-30.
   16. Lai C-C, Huang Y-C, Wei Y-H, Chang J-S. Biosurfactant-enhanced removal of total
   petroleum hydrocarbons from contaminated soil. J Hazard Mater. 2009;167:609–614.
   17. Saikia RR, Deka S, Deka M, Banat IM. Isolation of biosurfactant producing Pseudomonas
   aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in
   biosurfactant production. Annal. Microb.2012; 62:753–763.
   18. Lima TMS, Fonseca AF, Leão BA, Mounteer AH et al. Oil recovery from fuel oil storage tank
   sludge using biosurfactants. J Bio. Biodeg. 2011; 2:1-5.
   19. Amani H and Kariminezhad H. Study on emulsification of crude oil in water using emulsan
   biosurfactant for pipeline transportation. Pet. Sci. Tech. 2016; 34,216–222
   20. Mouafi F, Elsoud M, et al Optimization of biosurfactant production by Bacillus brevis using
   response surface methodology. biotech. Report.2016; 7: 1092
   21. Khademolhosseini R, Jafari A, Mosavi S, and et al. Physicochemical characterization and
   optimization of glycolipid biosurfactant production by a native strain of Pseudomonas
   aeruginosa HAK01 and its performance evaluation for the MEOR process. RSC Adv., 2019;
   9, 7932-7947
   22. Deepika, K. ., B.A. Kumar, S. Gnanender and P. . Bramhachari, Pseudomonas aeruginosa
   K D1 an efficient biosurfactant producing bacteria isolated from Krishna Delta Mangrove
   Sediments. Res. J. Environ. Sci.,2014, 8: 134-141
   23. Ghorbannezhad H, Moghimi H, Dastgheib M. Biodegradation of high-molecular-weight
   aliphatic and aromatic hydrocarbons by Aspergillus calidoustus, J of Micro.World.2018; 10:
   4,346-359.[ In Persian]