Isolation and molecular identification of Mycoplasma hominis from genital system of infertile men and women

Subject Areas : Medical Microbiology

Samaneh Jamalizadeh Bahaabadi ¹ , Babak Kheirkhah ² , Alireza Farsinezhad ³ , Victoria Habibzadeh ⁴

1 - MS.c., Department of Microbiology, Sirjan Science and Research Branch, Islamic Azad University, Sirjan, Iran.
2 - Assistant Professor, Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran.
3 - Assistant Professor, Department of Pathology, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran.
4 - Assistant Professor, Kerman University of Medical Sciences, Kerman, Iran.

Received: 2013-12-07 Accepted : 2015-03-01 Published : 2014-10-01

Keywords: infertility, 16S rRNA, Mycoplasma hominis, Molecular identification,

Abstract :

Background & Objectives: Mycoplasmas are one of the causative aetiologies of infertility in human beings. Genital Mycoplasmal infections can damage reproductive systems and lead to infertility infant mortalities. The aim of this study was molecular identification of Mycoplasma hominis isolated from genital system of infertile men and women in Kerman. Materials & Methods: This descriptive study was performed on 100 infertile men and 100 infertile women with a six month purposive sampling from the patients who referred to Infertility Center of Kerman. Semen and vaginal swab were tested by PCR assay for the presence of M. hominis. PCR product of positive samples were selected for sequencing. Sequence alignment was performed using MEGA 5 software and Neighbor-joining method. Results: In this study 45% of the samples taken from men were infected to Mycoplasma. Among them, 33% of the isolates belonged to M. hominis. Furthermore, 43% samples taken from women were infected to Mycoplasma, among them 41.8% belonged to M. hominis. Based on sequencing analysis, these M. hominis isolated from the patients were categorized into 5 different strains. Conclusion: In this study, M. hominis was indicated as the main microbial reason of infertility in this group. Sequence analysis of different M. hominis showed significant variability among the isolates. Therefore, these isolates can be reported as the local strains of Iran.