Isolation and molecular identification of Lysinibacillus macroides isolated from wheat roots and investigate their antifungal activity against Trichoderma spp.

Subject Areas : Plant Microbiology

Mahboobeh Choopan Vamerzani ¹ , Hamidreza Pordeli ² , Majid Moghbeli ³

1 - M.Sc., Department of Microbiology, Faculty of Biological Science, Islamic Azad University, Damghan branch, Damghan, Iran
2 - 2Assistant Professor, Department of Microbiology, Faculty of Biological Science, Islamic Azad University, Gorgan branch, Gorgan, Iran
3 - Assistant Professor, Department of Microbiology, Faculty of Biological Science, Islamic Azad University, Damghan branch, Damghan, Iran

Received: 2015-05-03 Accepted : 2015-09-15 Published : 2016-01-01

Keywords: 16srRNA, Lysinibacillus macroides, Endophytic, Antagonist,

Abstract :

Background & Objectives: Lysinibacillus is an endophytic bacterium that protects plant against environmental and physiological condition. The purpose of this study was to isolation and molecular identification of Lysinibacillus macroides from wheat roots and to analyse their antifungal activity against Trichoderma spp. Material & Methods: The extract of root and stem cultivated wheats in different areas of Mazandaran and Golestan were used for isolation of endophytic bacteria. Their antifungal activity was evaluated in a dual culture method on the Potato Dextros Agar (PDA) medium. After genomic DNA extraction, 16S rRNA gene was amplified using PCR. Then the PCR product was sequenced by BLAST. Results: The isolated bacteria were able to inhibit the growth of biocontrol fungi. Based on the 16srRNA sequence studies, this bacterium belonged to Lysinibacillus and showed 99% similarity to L. macroides 1w. Conclusion: This study is the first report of isolation of Lysinibacillus from the endophytic plants. The endophytic bacteria isolated in this study can be used for extraction, identification and application of its inhibitory metabolites for design and the production of antifungal agents.