Crude oil and polycyclic aromatic hydrocarbons (PAHs) biodegradation by Exophiala sp. UTMC 5043

Subject Areas : Microbial Biotechnology

Farahnaz  Akbarzadeh ¹ (M.Sc. student, Department of Biotechnology, Faculty of New Science and Technology, Semnan University, Semnan, Iran.)
Hamid  Moghimi ² (Assistant Professor, Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran.)
Shamsozoha  Abolmaali ³ (Assistant Professor, Faculty of Basic Science, Department of Biology, Semnan University, Semnan, Iran.)
Javad  Hamedi ⁴ (Professor, Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran.)

Received: 2017-09-12 Accepted : 2017-10-06 Published : 2018-08-23

Keywords: Bioremediation, Pyrene, Phenanthrene, Anthracene, Exophiala,

Abstract :

Background & Objectives: Today, crude oil products are one of the most widely used chemicals in the world. Daily, large volumes of crude oil and their derivatives are poured into the environment. The aim of this study was to isolate and evaluate Iranian indigenous yeast strains for the purpose of bioremediation of crude oil pollutants.Materials & Methods: Soil samples were collected from different oil-contaminated areas of Iran. The samples were cultured for 14 days on Bushnell Hass medium containing 0.5 % crude oil and 100 mg l-1 tetracycline. The crude oil degradation was measured by TPH assay at 420 nm. Removal of 100 ppm of phenanthrene, anthracene, and pyrene as model polycyclic aromatic hydrocarbons (PAHs) was studied using HPLC method. The superior yeast strain was identified by ITS gene sequencing and alignment in the NCBI database.Results: Totally, 47 yeast strains were isolated. TPH assay showed that FA14 isolate with 89% degradation rate within 14 days was the most powerful isolate in the removal of PAHs. ITS gene sequencing followed by alignment in NCBI indicated that FA14 belongs to the genus Exophiala sp., showing 99% similarity. Furthermore, the results indicated that within 14 days phenanthrene, anthracene, and pyrene were degraded by FA14 with the rate of 97.67 %, 57.0 %, and 95.38 %, respectively.Conclusion: Our results showed that Exophiala sp. has a high ability for biodegradation of crude oil and PAHs. Therefore, it could be introduced as a potent strain for bioremediation of oil-contaminated soil samples.

References:

1. Ron EZ, Rosenberg E. Enhanced bioremediation of oil spills in the sea. Curr Opin
   Biotecnol. 2014; 27:191-194.
   2. Mohanty S, Jasmine J, Mukherji S. Practical considerations and challenges involved in
   surfactant enhanced bioremediation of oil. BioMed Res Int. 2013; doi:10.115/2013/328608.
   3. Varjani SJ. Microbial degradation of petroleum hydrocarbons. Bioresour Technol. 2017; 223:
   277-286.
   4. Singh H. Mycoremediation: fungal bioremediation. John Wiley & Sons, Inc., Hoboken, New
   Jersey, USA; 2006.
   5. Mohsenzadeh F, Chehregani Rad A, Akbari M. Evaluation of oil removal efficiency and
   enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils. Iran J
   Environ Health Sci Eng. 2012; 9(1): 26-35.
   6. Singh A, Ward OP. Biodegradation and bioremediation. Springer-Verlag, Berlin, Heidelbberg;
   2004.
   7. Das N, Chandran P. Microbial degradation of petroleum hydrocarbon contaminants: an
   
   overview. Biotechnol Res Int. 2011; doi:10.4061/2011/941810.
   8. Gargouri B, Mhiri N, Karray F, Aloui F, Sayadi S. Isolation and characterization of
   hydrocarbon-degrading yeast strains from petroleum contaminated industrial wastewater.
   Biomed Res Int. 2015; doi: 10.1155/2015/929424.
   9. Kurtzman CP, Fell JW, Boekhout T. The yeasts: a taxonomic study. 5th Ed. Elsevier Science,
   Amesterdam; 2011.
   10. Eriksson M, Dalhammar G, Borg-Karlson AK. Biological degradation of selected
   hydrocarbons in an old PAH/creosote contaminated soil from a gas work site. Appl Micro
   Biotechnol. 2000; 53(5): 619-626.
   11. Pasumarthi R, Chandrasekaran S, Mutnuri S. Biodegradation of crude oil by Pseudomonas
   aeruginosa and Escherichia fergusonii isolated from the Goan coast. Mar Pollut Bull. 2013; 76
   (1): 276-282.
   12. Rinaldi MG. Use of potato flakes agar in clinical mycology. J Clinical Microb. 1982; 15(6):
   1159-1160.
   13. Rahman K, Thahira-Rahman J, Lakshmanaperumalsamy P, Banat IM. Towards efficient crude
   oil degradation by a mixed bacterial consortium. Bioresour Technol. 2002; 85(3): 257-261.
   14. Dritsa V, Rigas F, Natsis K, Marchant R. Characterization of a fungal strain isolated from a
   polyphenol polluted site. Bioresour Technol. 2007; 98(9): 1741-1747.
   15. Behnood M, Nasernejad B, Nikazar M. Biodegradation of crude oil from saline waste water
   using white rot fungus Phanerochaete chrysosporium. J Ind Eng Chem. 2014; 20(4): 1879-1885.
   16. Romero MC, Salvioli ML, Cazau MC, Arambarri AM. Pyrene degradation by yeasts and
   filamentous fungi. Env Pollution. 2002; 117(1): 159-163.
   17. Kamyabi A, Nouri H, Moghimi H. Synergistic effect of Sarocladium sp. and Cryptococcus sp.
   co-culture on crude oil biodegradation and biosurfactant production. Appl Biochem Biotechnol.
   2017; 182(1): 324-334.
   18. Srujana K, Khan AB. Isolation and characterization of polycyclic aromatic hydrocarbon
   degrading soil microbes from automobile workshop sediments. J Env Sci Technol. 2012; 5(1):
   74-83.
   19. Moghimi H, Tabar RH, Hamedi J. Assessing the biodegradation of polycyclic aromatic
   hydrocarbons and laccase production by new fungus Trematophoma sp. UTMC 5003. World J
   Microbiol Biotechnol. 2017; 33(7): 136.
   20. Bento FM, Camargo FA, Okeke BC, Frankenberger WT. Comparative bioremediation of soils
   contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation.
   Bioresour Technol. 2005; 96(9): 1055-1049.
   21. Atagana HI, Haynes R, Wallis F. Fungal bioremediation of creosote-contaminated soil: a
   laboratory scale bioremediation study using indigenous soil fungi. Water, air, soil Pollut. 2006;
   172(1): 201-219.
   
   22. Middelhoven WJ, Scorzetti G, Fell JW. Trichosporon veenhuisii sp. nov., an
   alkane-assimilating anamorphic basidiomycetous yeast. Int J Syst Evol Microb. 2000; 50(1):
   381-387.
   23. Assadirad MHA, Assadi MM, Rashedi H, Nejadsattari T. Isolation and phylogenetic analysis
   of indigenous oil-degrading bacteria from soil of Karoon area in Ahvaz. J Microb World. 2016;
   9(3): 236-246. [In Persian]
   24. Aranda, E. Promising approaches towards biotransformation of polycyclic aromatic
   hydrocarbons with Ascomycota fungi. Curr Opin Biotechnol. 2016; 38: 1-8.
   25. Zhang JH, Xue QH, Gao H, Ma X, Wang P. Degradation of crude oil by fungal enzyme
   preparations from Aspergillus spp. for potential use in enhanced oil recovery. J Chem Technol
   Biotechnol. 2016; 91(4): 865-875.
   26. Simister RL, Poutasse CM, Thurston AM, Reeve JL, Baker MC, White HK. Degradation of oil
   by fungi isolated from Gulf of Mexico beaches. Mar Pollut Bull. 2015; 100(1): 327-333.