Antibacterial effect of chitosan nanoparticles against Salmonella enteritidis

Subject Areas : Molecular Microbiology

Esmaeil Mahmoudi ¹ , Abbas Doosti ² , Mohammad-Saeid Jami ³

1 - M.Sc., Biotechnology Research Center, Shahrekord branch, Islamic Azad University, Shahrekord, Iran.
2 - Associate Professor, Biotechnology Research Center, Shahrekord branch, Islamic Azad University, Shahrekord, Iran.
3 - Assistant Professor, Biotechnology Research Center, Shahrekord branch, Islamic Azad University, Shahrekord, Iran.|Assistant Professor, Cellular and Molecular Research Center, Shahrekord University of Medical Science, Shahrekord, Iran.

Received: 2017-11-15 Accepted : 2017-11-27 Published : 2018-08-23

Keywords: Salmonella, Ionic gelation, Chitosan Nanoparticles,

Abstract :

Background & Objectives: Salmonella enteritidis causes a number of infections in humans and other animals. Though different antibiotics are used to eliminate bacterial infections, due to the development of antibiotic resistance after a while, the use of nanoparticles has been considered as suitable alternatives. Chitosan nanoparticles are appropriate options for the intended strategy due to some properties including low molecular weight and biodegradability. Therefore, the aim of this study was to evaluate the antibacterial effect of chitosan nanoparticles against S. enteritidis. Materials & Methods: Standard bacterial strain was prepared and subsequently confirmed by PCR technique. Ionic gelation method was used to fabricate chitosan nanoparticles and Hole-Plate and tube dilution methods were used to check the chitosan nanoparticles anti-microbial properties with antibiotics. At last Zeta's analysis techniques, dynamic optical scanning, and electron microscopy were used to evaluate nanoparticles. Results: A 214 base pair band confirmed the presence of bacteria. Chitosan nanoparticles with low molecular weight were produced by analyzing the results of optical dynamics scattering (111.7 nm), zeta analysis (20.8 mV) and microscopy (Conclusion: There is a significant relationship between the chitosan nanoparticles resistance and antibiotics against bacteria. In other words, the nanoparticles antibacterial properties were higher than antibiotics. It is deduced that chitosan nanoparticles can be used to control diseases and to destroy resistant bacterial species.

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