The effect of probiotic lactobacilli on the attachment power and biofilm formation of Escherichia coli isolated from urinary tract infections
Subject Areas : Medical Microbiology
Soudeh
Bandari
1
(دانشجوی کارشناسی ارشد، گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)
Nazila
Arbab Soleimani
2
(استادیار، گروه میکروب شناسی، دانشگاه آزاد اسلامی، واحد دامغان، دامغان، ایران)
Elahe
Tajbakhsh
3
(دانشیار، گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران)
Keywords: Escherichia coli, urinary infection, Anti-adhesive effect, Probiotic Lactobacilli,
Abstract :
Background & Objectives: Scientists are of the conviction that probiotic bacteria can ameliorate urinary tract infection by inhibiting colonization, attachment, and growth of uropathogenic Escherichia coli. The aim of this research was to investigate the anti-adhesive effect of two probiotic Lactobacilli including Lactobacillus plantarum and Lactobacillus casei on uropathogenic E. coli (UPEC).Materials & Methods: In research in 1395, 35 urinary tract infection (UTI) samples were collected from one hospital in Tehran and based on biochemical analysis 27 UPEC samples were identified. The ability of biofilm-formation and the presence of genes (papG and fimH) involved in biofilm- formation was investigated by microtiter plate and PCR methods, respectively. Co-aggregation and anti-adhesive effects of two probiotic lactobacilli including L. plantarum and L. casei against UPEC were studied by co-aggregation and microtiter plate method, respectively.Results: Among 27 isolates, 77%, 15%, and 5% showed strong, mediate and the weak ability of biofilm-formation, respectively, and 3% had no ability. Among 15 UPEC which had a strong biofilm-formation ability, 13 (86%) and 15 (100%) had papG and FimH genes, respectively. The average of co-aggregation between L. planetarium and L. casei with UPEC was gained 49.13% and 46.25%, respectively. The mean anti-adhesive effect of L. plantarium and L. casei against pathogenic bacterium was 62% and 58%, respectively.Conclusion: Further studies on the anti-adhesive effect of probiotic lactobacilli are suggested to prevent UPEC prevalence.
Escherichia coli. Exp Mol Pathol. 2008; 85: 11-19.
2. Maryam GS, Mohsen M, Shahin NP. Frequency of papaA, papC genes and antimicrobial
resistance pattern in uropathogenic Escherichia coli. J World Microbiol. 2016; 9(1): 44-52.
[In Persian]
3. Da SGJ, Mendonça N. Association between antimicrobial resistance and virulence in
Escherichia coli. Vir. 2012; 3(1): 18-28.
4. Lloyd AL, Henderson TA, Vigil PD, Mobley HL. Genomic islands of uropathogenic
Escherichia coli contribute to virulence. J Bacteriol. 2009; 1: 191(11): 3469-3481.
5. Ponnusamy PNV. In vitro biofilm formation by uropathogenic Escherichia coli and their
antimicrobial susceptibility pattern. Asian Pac J Trop Med. 2012; 5(3): 210-213.
6. Bermudez BM, Plaza DJ, QS M, LC G, Gil A. Probiotic mechanisms of action. Ann Nutr
Metab. 2012; 61: 160-174.
7. Hutt P, Shchepetova J, Loivukene K, Kullisaar T, Mikelsaar M. Antagonistic activity of
probiotic Lactobacilli and Bifidobacteria against entero and uropathogens. J App Microbiol.
2006; 100: 1324-1332.
8. Watson RR, Preedy VR. Probiotics, prebiotics, and synbiotics: Bioactive foods in health
promotion. 1st ed. Academic Press; 2015.
9. Sadri M, Arbab Soleimani N, Forghanifard MM. The study of antimicrobial and anti-adhesive
effect of probiotic Lactobacilli on uropathogenic Escherichia coli (UPEC). Biol J
Microorganism. 2016; 5(17): 159-170. [ In Persian]
10. Stepanovic S, Vukovic D, Hola V, Bonaventura GD, Djukic S, Cirkovic I, Ruzicka F.
Quantification of biofilm in microtiter plates: overview of testing conditions and practical
recommendations for assessment of biofilm production by staphylococci. Apmis. 2007; 115(8):
891-899.
11. Hojati Z, Zamanzad B, Hashemzade M, Molaie R, Gholipour A. The fimH gene in
uropathogenic Escherichia coli strains isolated from patients with urinary tract infection.
Jundishapur J Microbiol. 2015; 8(2): e17520.
12. Tajbakhsh E, Ahmadi P, Abedpour-Dehkordi E, Arbab-Soleimani N. Khamesipour F. Biofilm
formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli
isolated from clinical samples in Iran. Antimicrob Resist Infect Control. 2016; 5: 11.
13. Ershadian M, Arbab Soleimani N, Ajoudanifar H, Vaezi Khakhki MR. The antimicrobial and
co-aggregation effects of probiotic lactobacilli against some pathogenic bacteria. Iran J Med
Microbiol. 2015; 9(3): 14-22. [In Persian]
14. Sahoo TK, Jena P K, Nagar N, Patel AK, Seshadri S. In vitro evaluation of probiotic properties
of lactic acid bacteria from the gut of Labeo rohita and Catla catla. Probiotics Antimicrob
Proteins. 2015; 7(2): 126-136.
15. Abedi DF, Jafarian DA. In vitro anti- bacterial and anti-adherence effects of Lactobacillus
delbrueckii subsp bulgaricus on Escherichia coli. Res Pharm Sci. 2013; 8(4): 260-268.
16. Tapiainen T, Hanni AM, Salo J, Ikaheimo I, Uhari M. Escherichia coli biofilm formation and
recurrences of urinary tract infections in children. Eur J Clin Microbiol Infect Dis. 2014; 33:
111-115.
17. Kravchick S, Cytron S, Agulansky L, Bendor D. Acute prostatitis in middle-aged men: a
prospective study. Brit J Urol Int. 2004; 93(1): 93-96.
18. Amini Z, Arbab Soleimani N, Tajbakhsh E. Study of adhesive factor and biofilm formation of
uropathogenic Escherichia coli isolated from patient with urinary tract infection in Semnan
province. Pejouhandeh J. 2014; 6(96): 332-336. [In Persian]
19. Garofalo CK, Hooton TM, Martin SM, Stamm WE, Palermo JJ, Gordon JI. Escherichia coli
from urine of female patients with urinary tract infections is competent for intracellular
bacterial community formation. Infect Immun. 2007; 75: 52-60.
20. Tarchouna M, Ferjani A, Ben SW, Boukadida J. Distribution of uropathogenic virulence genes
in Escherichia coli isolated from patients with urinary tract infection. Int J Inf Dis. 2013; 17:
450-453.
21. Giray B, Ucar FB, Aydemir SS. Characterization of uropathogenic Escherichia coli strains
obtain ro urology out ati nt clinic o g ical aculty in İz ir. ur J ci. ;
42: 1328-1337.
22. Sabina F. Microorganisms with claimed probiotic properties: An overview of recent
literature. Int J Environ Res Public Health. 2014; 11(5): 4745-4767.
_||_
Escherichia coli. Exp Mol Pathol. 2008; 85: 11-19.
2. Maryam GS, Mohsen M, Shahin NP. Frequency of papaA, papC genes and antimicrobial
resistance pattern in uropathogenic Escherichia coli. J World Microbiol. 2016; 9(1): 44-52.
[In Persian]
3. Da SGJ, Mendonça N. Association between antimicrobial resistance and virulence in
Escherichia coli. Vir. 2012; 3(1): 18-28.
4. Lloyd AL, Henderson TA, Vigil PD, Mobley HL. Genomic islands of uropathogenic
Escherichia coli contribute to virulence. J Bacteriol. 2009; 1: 191(11): 3469-3481.
5. Ponnusamy PNV. In vitro biofilm formation by uropathogenic Escherichia coli and their
antimicrobial susceptibility pattern. Asian Pac J Trop Med. 2012; 5(3): 210-213.
6. Bermudez BM, Plaza DJ, QS M, LC G, Gil A. Probiotic mechanisms of action. Ann Nutr
Metab. 2012; 61: 160-174.
7. Hutt P, Shchepetova J, Loivukene K, Kullisaar T, Mikelsaar M. Antagonistic activity of
probiotic Lactobacilli and Bifidobacteria against entero and uropathogens. J App Microbiol.
2006; 100: 1324-1332.
8. Watson RR, Preedy VR. Probiotics, prebiotics, and synbiotics: Bioactive foods in health
promotion. 1st ed. Academic Press; 2015.
9. Sadri M, Arbab Soleimani N, Forghanifard MM. The study of antimicrobial and anti-adhesive
effect of probiotic Lactobacilli on uropathogenic Escherichia coli (UPEC). Biol J
Microorganism. 2016; 5(17): 159-170. [ In Persian]
10. Stepanovic S, Vukovic D, Hola V, Bonaventura GD, Djukic S, Cirkovic I, Ruzicka F.
Quantification of biofilm in microtiter plates: overview of testing conditions and practical
recommendations for assessment of biofilm production by staphylococci. Apmis. 2007; 115(8):
891-899.
11. Hojati Z, Zamanzad B, Hashemzade M, Molaie R, Gholipour A. The fimH gene in
uropathogenic Escherichia coli strains isolated from patients with urinary tract infection.
Jundishapur J Microbiol. 2015; 8(2): e17520.
12. Tajbakhsh E, Ahmadi P, Abedpour-Dehkordi E, Arbab-Soleimani N. Khamesipour F. Biofilm
formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli
isolated from clinical samples in Iran. Antimicrob Resist Infect Control. 2016; 5: 11.
13. Ershadian M, Arbab Soleimani N, Ajoudanifar H, Vaezi Khakhki MR. The antimicrobial and
co-aggregation effects of probiotic lactobacilli against some pathogenic bacteria. Iran J Med
Microbiol. 2015; 9(3): 14-22. [In Persian]
14. Sahoo TK, Jena P K, Nagar N, Patel AK, Seshadri S. In vitro evaluation of probiotic properties
of lactic acid bacteria from the gut of Labeo rohita and Catla catla. Probiotics Antimicrob
Proteins. 2015; 7(2): 126-136.
15. Abedi DF, Jafarian DA. In vitro anti- bacterial and anti-adherence effects of Lactobacillus
delbrueckii subsp bulgaricus on Escherichia coli. Res Pharm Sci. 2013; 8(4): 260-268.
16. Tapiainen T, Hanni AM, Salo J, Ikaheimo I, Uhari M. Escherichia coli biofilm formation and
recurrences of urinary tract infections in children. Eur J Clin Microbiol Infect Dis. 2014; 33:
111-115.
17. Kravchick S, Cytron S, Agulansky L, Bendor D. Acute prostatitis in middle-aged men: a
prospective study. Brit J Urol Int. 2004; 93(1): 93-96.
18. Amini Z, Arbab Soleimani N, Tajbakhsh E. Study of adhesive factor and biofilm formation of
uropathogenic Escherichia coli isolated from patient with urinary tract infection in Semnan
province. Pejouhandeh J. 2014; 6(96): 332-336. [In Persian]
19. Garofalo CK, Hooton TM, Martin SM, Stamm WE, Palermo JJ, Gordon JI. Escherichia coli
from urine of female patients with urinary tract infections is competent for intracellular
bacterial community formation. Infect Immun. 2007; 75: 52-60.
20. Tarchouna M, Ferjani A, Ben SW, Boukadida J. Distribution of uropathogenic virulence genes
in Escherichia coli isolated from patients with urinary tract infection. Int J Inf Dis. 2013; 17:
450-453.
21. Giray B, Ucar FB, Aydemir SS. Characterization of uropathogenic Escherichia coli strains
obtain ro urology out ati nt clinic o g ical aculty in İz ir. ur J ci. ;
42: 1328-1337.
22. Sabina F. Microorganisms with claimed probiotic properties: An overview of recent
literature. Int J Environ Res Public Health. 2014; 11(5): 4745-4767.
