Engineering of lipid production in oleaginous microorganisms
Subject Areas : Microbial BiotechnologyFateme Ghanaatian 1 , Ahmad Farhad Talebi 2
1 - M.Sc. student, Faculty of Biotechnology, Semnan University, Semnan, Iran.
2 - Assistant Professor, Faculty of Biotechnology, Semnan University, Semnan, Iran.
Keywords: Biodiesel, lipid metabolism, Genetic Engineering, Oily microorganisms, Microbial oils,
Abstract :
Microbial oils are of great interest to researchers as they provide essential fatty acids and are among renewable energy sources. Oleaginous microorganisms have the ability to produce oils up to 60% of their weight, most of which are accumulated in the form of triglycerides. Fouroleaginous genera including bacteria, microalgae, fungi, and yeasts are among the largestproducers of microbial oils. A variety of physical and chemical factors are effective in microbial oils production, among which carbon source, nutrient deficiency, temperature, light intensity and pH of the environment can be noticed. Considering the constraints faced by various physical and chemical treatments in microbial oils production, most researches are currently focusing ongenetic modifications to enhance lipid production in oleaginous microorganisms. This articlereviewed 210 peer-reviewed articles entitled engineering of lipid production in oleaginousmicroorganisms in scientific databases during 1990-2017, among which 89 to particles wereselected for further assessments. In order to have the best achievement in replacement of new oil sources on a large scale, these resources should be optimized in terms of unique properties such as unsaturated fatty acids production, lipid yield, as well as lipid profile. The present study is anattempt to recommend some basic approaches to increase microbial oils production by oleaginous microorganisms. These strategies should be in line with genetic and environment-protection priorities. Concurrently, they could be useful for knowledge-based enterprises from the economical point of view.
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_||_
Yen HW, Hu IC, Chen CY, Ho SH, Lee DJ, Chang JS. Microalgae-based biorefinery from
biofuels to natural products. Bioresour Technol. 2013; 135: 166-174.
2. Talebi AF, Mohtashami SK, Tabatabaei M, Tohidfar M, Bagheri A, Zeinalabedini M, Mirzaei
H, Mirzajanzadeh M, Shafaroudi S, Bakhtiari S. Fatty acids profiling: a selective criterion for
screening microalgae strains for biodiesel production. Algal Res. 2013; 2(3): 258-267.
3. Liang MH, Jiang JG. Advancing oleaginous microorganisms to produce lipid via metabolic
engineering technology. Prog Lipid Res. 2013; 52(4): 395-408.
4. Bellou S, Triantaphyllidou IE, Aggeli D, Elazzazy AM, Baeshen MN, Aggelis G. Microbial
oils as food additives: recent approaches for improving microbial oil production and its
polyunsaturated fatty acid content. Curr Opin Biotechnol. 2016; 37: 24-35.
5. Talebi AF, Tohidfar M, Mousavi Derazmahalleh SM, Sulaiman A, Baharuddin AS, Tabatabaei
M. Biochemical modulation of lipid pathway in microalgae Dunaliella sp. for biodiesel
production. Biomed Res Int. 2015; 2015: 1-12.
6. Donot F, Fontana A, Baccou J, Strub C, Schorr-Galindo S. Single cell oils (SCOs) from
oleaginous yeasts and moulds: production and genetics. Biomass Bioenergy. 2014; 68:
135-150.
7. Adrio JL. Oleaginous yeasts: promising platforms for the production of oleochemicals and
biofuels. Biotechnol Bioeng. 2017; 144(19): 1915-1920.
8. Beopoulos A, Nicaud J-M, Gaillardin C. An overview of lipid metabolism in yeasts and its
impact on biotechnological processes. Appl Microbiol Biotechnol. 2011; 90(4): 1193-1206.
9. Bazinet RP, Layé S. Polyunsaturated fatty acids and their metabolites in brain function and
disease. Nat Rev Neurosci. 2014; 15(12): 771-778.
10. Bruen R, Fitzsimons S, Belton O. Atheroprotective effects of conjugated linoleic acid. Br J
Clin Pharmacol. 2017; 83(1): 46-53.
11. Passoth V. Lipids of yeasts and filamentous fungi and their importance for biotechnology.
Biotechnology of Yeasts and Filamentous Fungi: Springer; 2017; 83(1): 149-204.
12. ača , arcinčá , Čertí , pel a , arcinčá á , t á , lnár ,
lemp á , as aľ á . ffect f a in prefrmente cereal pr ct c ntainin
gamma-linolenic acid to broiler feed on production indicators and fatty acid profile of chicken
breast. Acta Ve Brno. 2014; 83(4): 379-384.
13. Madani M, Enshaeieh M, Abdoli A. Single cell oil and its application for biodiesel
production. Process Saf Environ. 2017; 92(3): 46-53.
14. Li Q, Du W, Liu D. Perspectives of microbial oils for biodiesel production. Appl Microbiol
Biotechnol. 2008; 80(5): 749-756.
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J Biosci Bioeng. 2006; 101(2): 87-96.
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17. Shaojin Y, Yiping Z. Research and application of oleaginous microorganism. China
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Bioprocess Engin. 2005; 3(1): 23-27.
19. Gouda MK, Omar SH, Aouad LM. Single cell oil production by Gordonia sp. DG using
agro-industrial wastes. World J Microbiol Biotechnol. 2008; 24(9): 1703-1659.
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