A Consideration on against antibiotic and heavy metals resistance in isolated bacteria from intestine organ of Anzali Lagoon’s Cyprinus Carpio
Subject Areas : Marine BiologySh. Safaeian 1 , H. Hosseiny 2 , A. Esmaili 3 , Z. Moghaddam 4 , H. Vakili َAmini 5
1 - Faculty of Marine Science & Technology, Islamic Azad University, North Tehran Branch
2 - Faculty Board of Foodstuffs & Medicine Research Center of the Ministry of Health, State Foodstuff Industries and Nourishment Research Institute, Sheheed Beheshti University of Medical Sciences
3 - Faculty of Chemistry, Islamic Azad University, North Tehran Branch
4 - Faculty of Marine Science & Technology, Islamic Azad University, North Tehran Branch
5 - Faculty of Marine Science & Technology, Islamic Azad University, North Tehran Branch
Keywords: Antibiotic resistance, Anzali lagoon, Bacterium, Heavy Metal Resistance, Common Carps,
Abstract :
Sampling of wild Common carps from Anzali Lagoon was operated in winter and spring 2009 in order to modify resistance of bacteria of their intestinal organ against antibiotics and heavy metals and sensitivity of all bacteria to 6 antibiotics of ampicillin, streptomycin, tetracycline, cloramphenicl, gentamicin and amikacin was considered. The results show that isolated bacteria had high resistance against three antibiotics of Ampicillin (92.92%), Streptomycin (45.14%) and Tetracycline (45.73%) but that they had approximately low resistance against Cloramphenicl (12.82%). According to the results Common carps had no resistance against two other antibiotics of Gentamicin (0%) and Amikasin (0%). The Isolated bacteria from intestinal organ of Common carps also showed high level of multi-resistance patterns against above mentioned antibiotics. Sample bacteria were also considered for their resistance against three heavy metals of copper, cadmium and mercury. According to researches the bacteria from intestinal organ resistance against heavy metals with different densities (100 and 200 μg/ml) and the resistance pattern was figured as Cu2+>Cd2+>Hg2+. Studying the solidarity between heavy metal resistance and antibiotic resistance in Isolated the bacteria from intestinal organ of Common carps by Person Test, the linear relation between the percentage of resistant bacteria against heavy metals and percentage of resistant bacteria against antibiotics was achieved. This achievement confirms that increasing of metal pollutions increases the number of resistant bacteria against antibiotics and heavy metals in Anzali Lagoon. This study is executed for the first time in Anzali Lagoon and shows the probability of antibiotic resistant bacteria metastasis to human by having fishes. This can be a serious subject which threatens general health of society.
