A study on effects of mutagenicity of muscle, cartilage and liver of Persian Gulf shark (Sphyrena lewini) , using the bacterium Salmonella thyphimurium

Subject Areas : Marine Biology

M. Emtiazjoo ¹ , L. Salimi ² , S. Samadi ³ , F. Hayati ⁴

1 - Faculty of Marine Science and Technology, Islamic Azad University, North Tehran Branch
2 - Faculty of Marine Science and Technology, Islamic Azad University, North Tehran Branch
3 - Faculty of Marine Science and Technology, Islamic Azad University, North Tehran Branch
4 - Faculty of Marine Science and Technology, Islamic Azad University, North Tehran Branch

Received: 2010-08-09 Accepted : 2010-09-02 Published : 2010-12-21

Keywords: Salmonella typhimurium, Muscle, Gallbladder, Antimutagenesis, Ames test, Shark, cartilage. Persian Gulf,

Abstract :

Shark cartilage, liver and muscle may have an inhibitory effect on mutagenicity and accordingly there is a correlation between antimutagenesis and anticarcinogenesis. The use of antimutagens and anticarcinogens in everyday life is the most effective procedure to preventing human cancer and genetic diseases. In this research, we studied the effects of cartilage, muscle and liver extracts of family Sphyrnidae in Persian Gulf on antimutagenicity and antioxidant by Ames test, using Salmonella typhimurium strains in the second phase, especially in the presence and absence of liver microsomal fraction (S9). The number of revertant colonies was compared with negative and positive controls. The results showed that, percentage of antimutagenic and antioxidant effect of cartilage was around 90%. Cartilage extract showed stronger antimutagenic effect in comparison with muscle and liver extracts.Antimutagenic substances may prevent cancer because they can destroy mutagens both inside and outside body cells, and block mutagens that damage DNA and cause mutation in cells. These extracts exhibited an antimutagenic activity that could be beneficial in late as well as early stages of cancer development