Detection of Histamine level and relation with microbial load of whole Tiger croaker (Otolithes ruber) during ice storage

Subject Areas : Marine Biology

A. Sotoodeh ¹ (Faculty of Marine Science and Technology, North Tehran Branch, Islamic Azad University)
S. Moini ² (Faculty of Marine Science and Technology, North Tehran Branch, Islamic Azad University)
P. Nejatkhah Manavi ³ (Faculty of Marine Science and Technology, North Tehran Branch, Islamic Azad University)

Received: 2008-12-24 Accepted : 2009-03-08 Published : 2009-06-21

Keywords: Otolithes ruber, putrescine, Biogenic amine, Microbial load, ice storage,

Abstract :

In this study, the biogenic amine Histamine (BA) content of whole croaker(Otolithes ruber) was monitored during ice storage for a period of 18 days and related bacterial changes (psychrophiles and mesophiles) were studied every 3 days (0, 3, 6, 9, 12, 15 and 18th day ). Histamine was not detected in the first storage day by HPLC and this amine detected after 5 days. The best correlation (R2=0.98) were found among Histamine and mesophilic bacterial load also showed significant variation (P<0.05) during ice storage. Initial concentration of Histamine was 1.49µg/g and finally reached to 14.21 µg/g when mesophilic bacterial load reached over 107cfu/g. The level of Histamine seemed to be a suitable indicator freshness of Otolithes ruber.