Synthesis, characterization, and evaluation of antibacterial activities of novel nanocomposite films chitosan/phosphorus triamide/Fe3O4 NPs

Subject Areas :

samad yarahmadi ¹ , niloufar dorosti ² , maryam pas ³ , abdul naser mohammadi ⁴

1 - M.Sc. Student of Inorganic Chemistry, Faculty of Chemistry, Lorestan University, Khorramabad, Iran.
2 - Associate Prof. of Inorganic Chemistry, Inorganic Chemistry Department, Faculty of Chemistry, Lorestan University, Khorramabad, Iran.
3 - Ph.D Student of Inorganic Chemistry, Faculty of Chemistry, Lorestan University, Khorramabad, Iran.
4 - Assistant Prof. of Department of Biology, Faculty of Basic Sciences, Lorestan University, Khorramabad, Iran

Received: 2023-01-27 Accepted : 2023-04-03 Published : 2023-05-22

Keywords: Antibacterial, Chitosan, nanocomposites, Fe3O4 NPs, Phosphorus triamide,

Abstract :

In this work, new nano-composite of chitosan/phosphorus triamide with Fe3O4 NPs (1, 2.5, 5%) was prepared. The Fe3O4 NPs were synthesized at size of about 18-24 nm with a spherical morphology through co-precipitation method by using FeCl3.9H2O and FeCl2.4H2O salts. The phosphorus triamide derivative was synthesized from the reaction of tert-butylamine with phosphoryl chloride. X-ray diffraction (XRD) patterns and scanning electron microscope (SEM) images of chitosan, phosphorus triamide, Fe3O4 NPs, chitosan/phosphorus triamide, and chitosan/phosphorus triamide/1-5% Fe3O4 NPs were investigated and the results confirmed the formation of the desired films. Further, morphology and the size of nanoparticles were investigated by changing ultrasonic frequency (37 and 80 Hz) and power (30, 60, and 100 W). The in vitro antibacterial activities were evaluated against two gram-positive Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus) and one gram-negative Pseudomonas aeruginosa (P. aeruginosa) bacteria. Nanocomposite containing higher percent of Fe3O4 NPs showed more antibacterial activities. Results also displayed greater antibacterial effects against B. cereus bacterium. 

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