Investigation of the effect of quercetin on the survival rate of MCF-7 breast cancer cells by MTT and Trypan blue assays

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

fatemeh zohreh ¹ , kazem parivar ² , nasim Hayati Roudbari ³ , parichehr yaghmaei ⁴

1 - Department of biology, Science and Research branch, Islamic Azad University, Tehran, Iran
2 - Science and Research Branch, Daneshgah Blvd, Simon Bulivar Blvd, Tehran, Iran
3 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - biology,basic science,science and research branch of the Islamic Azad university

Received: 2022-05-28 Accepted : 2022-08-09 Published : 2023-11-22

Keywords: breast cancer, MTT Assay, Quercetin, MCF-7, HEK,

Abstract :

In this study, the effect of quercetin on the survival of cancer cells was investigated using MTT and trypan blue tests. Quercetin is a flavonol that has antioxidant properties and is found in fruits, vegetables, and especially colored seeds. Breast cancer cells (MCF-7 cell line) and healthy cells (HEK-293 cell line) were exposed to 50 and 100 micromolar doses of quercetin for 24 and 48 hours.To check the viability of cancer cells, MTT and trypan blue tests were performed. According to the results, the survival rate of cancer cells with Q50 μM dose in 48 hours was significantly decreased in the surface (P < 0.01). Also, a significant decrease was observed in Q100 μM dose at 24 and 48 hours. This decrease was significant at 24 hours at P < 0.05 and at 48 hours at P < 0.01. The results of tests showed the positive anti-cancer effects of quercetin. Based on the results of this research, quercetin reduced the survival rate of cancer cells and the best result was observed in 48 hours. Considering that the anti-cancer effects of quercetin were greater after 48 hours, long-term use of this drug is recommended to achieve its therapeutic effects.

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