Investigating different methods to identify genotypes of species of the genus Armillaris (honey mushroom
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
1 - 2. Department of Biology, Faculty of Biology Science, Islamic Azad University, North Tehran Branch, Tehran, Iran
Keywords: RFLP, RAPD, Species of the genus Armillaris, type-mixing alleles,
Abstract :
The purpose of the recent study is to compare SI with three other methods for distinguishing genotypes, which are: DNA fragment polymorphism method resulting from random multiplication (RAPD), investigation of mixed-type alleles and analysis of RAPD isoenzymes and type-type alleles. A mixture of 73 isolates, belonging to 36 genotypes identified based on the analysis, were used. A limited number of isolates were investigated using isozyme polymorphism. RAPDs, mixed-type alleles and SI in all three cases of isolates differentiated through SI reactions included two subgroups with different RAPD phenotypes.In two cases, the groups differentiated through SI reactions shared a similar set of alleles of the mixing type. Isoenzyme analysis showed interspecies differences and insignificant differences between genotypes of the same species. These findings have led to consider the use of SI as a routine and valid method for epidemiological studies of Armillaria species.In the same group of isolates, profiles of the EcoR I fragment related to mitochondrial DNA were also investigated. This analysis regrouped the genotypes into 'mitochondrial types' that share common cytoplasm and are likely closely related through sexual reproduction.
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