The effect of microbial phytase enzymes Hostazim, Physaem and Ronosyme on expression of IGF1 functional gene in broilers
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Kian
Pahlevan Afshari
1
(Department of Animal Science, Faculty of Agriculture, Varamin pishva Branch, Islamic Azad University, Varamin, Iran)
Hamid Reza
Seyed Abadi
2
(Animal Science Research Institute of Iran, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran)
Ali
Esmailpoor
3
(Msc of Animal Science,Department of Animal Science, Faculty of Agriculture, Abhar Branch, Islamic Azad University, Abhar, Iran)
Keywords: broilers, IGF-1, Phytases,
Abstract :
The amount of insulin-like growth factor (IGF) is regulated by various stimuli such as hormones, growth factors and nutritional conditions. In the present study, the effect of different levels of phytase enzymes on the expression of IGF-1 gene in the liver tissue of broiler chickens was investigated. The experiment was conducted with 7 treatments, 5 repetitions and 25 chicken pieces in each repetition. Experimental diets including the control diet, whose required phosphorus was provided by dicalcium phosphate, and the diets containing different commercial microbial phytase enzymes based on corn and soy were investigated. In order to investigate the expression of IGF-1 gene, first, total RNA was extracted from liver tissue and after making cDNA, the gene expression level was measured using real time PCR technique. The results of examining IGF-I gene expression in different levels of phytases showed a significant difference between the treatments. The expression of IGF-I gene in treatments 1 and 2 including phytase hostazim and phyzyme showed the lowest expression level and treatment 6 with phytases hostazim and ranozyme 100% showed the highest expression level. Increasing the amount of phytases increases the expression of IGF1-mRNA in the liver and can subsequently affect the performance of growth traits.
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