Influence of air pollution in part of Arak city on anatomical structure of spinach (Spinacea oleracea L.)

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

zohreh  jafari ¹ (Assistant professor, PhD in biology, Department of biology,Arak Branch, Islamic Azad university, Arak, Iran.)
Maryam  Dehghan ² (Department of biology, North Tehran Branch, Islamic Azad university, Tehran, Iran)

Received: 2017-10-09 Accepted : 2018-04-09 Published : 2017-11-22

Keywords: air pollution, spinach, anatomical structure,

Abstract :

Background and Purpose: Spinach (Spinacia oleracea L.) is an annual plant belonging to the Chenopodiace family, whose cultivation history reaches two thousand years in Iran. The idea of this study was to investigate changes in the structure of spinach plant anatomy in the dense part of the contaminated air Arak city. Analysis method: Spinach seed samples simultaneously in two groups of control and treated, In two parts with clean air (Haftadgholeh Arak National Park )and condensation(Iralko Park Aluminum Factory) from air pollution in the city were planted. After growing Cotyledons leaves and Original leaves , the plant specimens were fixed in Alcohol -Gycerin solution . After sectioning and double coloring with Methylene blue and Zaghic Carmen, specimens were photographed by Photomicroscope then anatomical structure Changes were studied. Result and discussion: The changes in the anatomical structure of leaves of spinach showed that in samples that were grown in the dense portion of the city Compared to the control samples, had thicker cuticle and the structure of protective trichomes were abundant. Chlorenchyma have been irregular and stored parenchyma were full of Calcium oxalate crystal deposition. Finally, we can conclude that Plant samples of the air polluted city were stressed by air pollution which is likely have adversed effects on the quality of plant food.

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