Influence of air pollution in part of Arak city on anatomical structure of spinach (Spinacea oleracea L.)

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

zohreh jafari ¹ , Maryam Dehghan ²

1 - Assistant professor, PhD in biology, Department of biology,Arak Branch, Islamic Azad university, Arak, Iran.
2 - Department of biology, North Tehran Branch, Islamic Azad university, Tehran, Iran

Received: 2017-10-09 Accepted : 2018-04-09 Published : 2017-11-22

Keywords: air pollution, spinach, anatomical structure,

Abstract :

Background and Purpose: Spinach (Spinacia oleracea L.) is an annual plant belonging to the Chenopodiace family, whose cultivation history reaches two thousand years in Iran. The idea of this study was to investigate changes in the structure of spinach plant anatomy in the dense part of the contaminated air Arak city. Analysis method: Spinach seed samples simultaneously in two groups of control and treated, In two parts with clean air (Haftadgholeh Arak National Park )and condensation(Iralko Park Aluminum Factory) from air pollution in the city were planted. After growing Cotyledons leaves and Original leaves , the plant specimens were fixed in Alcohol -Gycerin solution . After sectioning and double coloring with Methylene blue and Zaghic Carmen, specimens were photographed by Photomicroscope then anatomical structure Changes were studied. Result and discussion: The changes in the anatomical structure of leaves of spinach showed that in samples that were grown in the dense portion of the city Compared to the control samples, had thicker cuticle and the structure of protective trichomes were abundant. Chlorenchyma have been irregular and stored parenchyma were full of Calcium oxalate crystal deposition. Finally, we can conclude that Plant samples of the air polluted city were stressed by air pollution which is likely have adversed effects on the quality of plant food.

References:

[1]. Assadi, A., A. G. Pirbalouti, F. Malekpoor, N. Teimori, and L. Assadi, "Impact of air pollution on physiological and morphological characteristics of Eucalyptus camaldulensis," J. Food Agric. Environ, vol. 9, pp. 676-679, 2011.

[2]. Asadi, and Hasandokht, M.R., " Study the genetic variation of genotypes spinach," IRANIAN JOURNAL OF AGRICULTURAL SCIENCES (JOURNAL OF AGRICULTURE), vol. 38, pp. 257-265, 2005.

[3]. Amini, F., N. Fatah ravandi, and M. Askari, "Anatomical Study of the Air Pollution Effect on Robinia pseudoacacia and Ailanthus altisima leaves near to Iran Aluminum Co. (IRALCO)," Journal of Cell & Tissue, vol. 6, pp. 501-511, 2016.

[4]. Bastani Safdarabadi, F. M. Mosaedi, M., A., "Investigating the Effects of Heavy Metals Pollution on Stachys Trinervis and Subsoil Soils in the Bojnourd Cement Factory," in The 6th National Conference on Rangeland and Standards of Iran, Mashad university, 2015.

[5]. Baghaei. AH, "Effect of tire rubber ash enriched municipal waste compost on decreasing spinach Cd concentration (a case study: Arak municipal waste compost," Iranian Journal of Health and Environment, vol. 10, pp. 401-410, 2017.

[6]. Darvishsefat, A. Atlas, A., of protected areas of Iran: Ravi, 2006.

[7]. Delphieh. M., H. S., and S. D. F., "The Effect of Adding Spiolite and Paligurskite Minerals to an Contaminated Soil on Cadmium and Lead Elevated by Spinach," IRANIAN JOURNAL OF SOIL RESEARCH (FORMERLY SOIL AND WATER SCIENCES), vol. 29, pp. 436-443, 2015.

[8]. Erfani, F. Hasandokht,M. BARZEGAR, M., and JABARI, A., "Determination and comparison of chemical properties of seven Iranian spinach cultivars," 2006.

[9]. Jafari, N. M. Eshghi-Malayeri, M, B. , "Studies of fluorides on vegetation of Iranian aluminium company area and their accumulation in Medicago sativa," Findings Sci., vol. 7(4), pp. 1-9., 2011.

[10]. Shaban khani, Â. b. M. Shokri lomohi, B, M, Bahrami S., "Qualitative Analysis of lead and cadmium in Spinach and Radish, in Sari, in autumn 1999," Journal of Mazandaran University of Medical Sciences, vol. 11, pp. 27-30, 2001.

[11]. Singsaas, E., D. Ort, and DeLucia, E., "Elevated CO2 effects on mesophyll conductance and its consequences for interpreting photosynthetic physiology," Plant, Cell & Environment, vol. 27, pp. 4150, 2004.

[12]. Pirsaheb, M., Ahmadian, M., Reshadat, S., and Poormohammadi, A., "The Measurement of Nitrite and Nitrate Contents of Tomato from the Vast Plain of Kermanshah State of Iran," International Journal of Agriculture and Crop Sciences, vol. 7, p. 1553, 2014.

[13]. RASHIDI, F., JALILI, A., BABAIE, K. S., and T. K. SAGHEB, "RESPONSE OF LEAF ANATOMY IN ASH (FRAXINUS ROTUNDIFOLIA MILL.) TO POLLUTANT GASES AND CLIMATIC FACTORS," 2011.

[14]. Rechinger, K. "Flora iranica: no. 157. Papilionaceae: 2," Graz: Akademische Druck-und Verlagsanstalt 499p.-keys.. Ge Many new taxa. Geog, vol. 2, pp. 259-272., 1963-1988.

[15]. Ronaghi. A., P. Y., and K. N., "Effect of Nitrogen and Manganese on Growth and Chemical Composition of Spinach," THE IRANIAN JOURNAL OF BOTANY, vol. 5, pp. 71-83, 2001.

[16]. ZAVVAR, M. H., A. ROOHALLAHI, H. SHIRKHANLOO, and E. ALAIE, "THE INVESTIGATION OF ENVIRONMENTAL CONTAMINATING EFFECT OF SHARE-REY PETROLEUM COMPLEX ON REGIONAL PLANTS," 2008.

[17]. Zarinkamar, F., "FOLIAR ANATOMY OF CHENOPODIACEAE FAMILY AND XEROPHYTES ADAPTATION," 2006.

[18]. Sanaeirad, H. S, Majd, A., Abbaspour, H. and M. Peyvandi, "The Relationship Between Air Pollutionand Anatomical and Development of the Laurus nobilis L. Plant and Two Effective Enzymes on This Relationship," Developmental Biology, vol. 8, pp. 59-73, 2016.