Investigating of Ultrastructure and Histology Leaf and Root of castor seeds (Ricinus communis L.) under concentrations of multi-walled carbon nanotubes

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Zahra Fathi ¹ ( Department of Biology, Faculty of Science,Science and Research Branch, Islamic Azad University, Tehran, )
Ramazan Ali Khavari-Nejad ² ( Department of Biology, Faculty of Science,Science and Research Branch, Islamic Azad University, Tehran, Iran )
Homa Mahmoodzadeh ³ ( Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran )
Taher Nejad-Sattari ⁴ ( Department of Biology, Faculty ofScience,Science and Research Branch, Islamic Azad University, Tehran, Iran )

Received: 2018-01-11 Accepted : 2018-05-22 Published : 2018-05-22

Keywords: TEM, XRD, MWCNT, Ricinus plant, Ultrastructuer of leaf and root,

Abstract :

The castor plant )Ricinus communis L.) belongs to the Euphorbiaceae family and is a very important medicinal plant. Carbon nanotubes act as regulator of germination and growth of plant; and are able to change the morphology and physiology of plant cell.Penetration of carbon nanotubes in plant systems are able to change metabolic acts that lead to increase products . XRD and nanoparticle sizer experiments showed that the major phase is carbon and its size is range of 35-300 nm. The aim of this study is to investigate the effect of concentrations of multi-walled carbon nanotubes (MWCNTs) (0 and 500 µg ml-1 ) alongside the control under laboratory conditions on the characters of histology and ultrastructure. The vegetative growth was done in greenhouse conditions .foliar spray 500 µg ml-1 treatment (MWCNTs and deionezed water for 0 treatment) was done. Duration of experiment was 45 days. after that plant was exited from soil, shoot and root were seprated.process of preparation was done and samples go over on grid for study TEM.The result of ultra strcture studies of root and leaf showed, there is great aggregation of multi-walled carbon nanotubes with different length in vacuole and cytoplasm which in leaf cells have aggregation of vacuole and increase of plastogloboly in stroma of chlroplast and transformation of chloroplast from globular to ellipsoid and in root cells lead to destruction of mitochondria crista, vacoule and nucleus membrane and low chromatins, also was observed aggregation of vacuole and many diameter of sclreid and xylem wall.

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