Investigating of Ultrastructure and Histology Leaf and Root of castor seeds (Ricinus communis L.) under concentrations of multi-walled carbon nanotubes

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Zahra Fathi ¹ , Ramazan Ali Khavari-Nejad ² , Homa Mahmoodzadeh ³ , Taher Nejad-Sattari ⁴

1 - Department of Biology, Faculty of Science,Science and Research Branch, Islamic Azad University, Tehran,
2 - Department of Biology, Faculty of Science,Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
4 - Department of Biology, Faculty ofScience,Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2018-01-11 Accepted : 2018-05-22 Published : 2018-05-22

Keywords: TEM, XRD, MWCNT, Ricinus plant, Ultrastructuer of leaf and root,

Abstract :

The castor plant )Ricinus communis L.) belongs to the Euphorbiaceae family and is a very important medicinal plant. Carbon nanotubes act as regulator of germination and growth of plant; and are able to change the morphology and physiology of plant cell.Penetration of carbon nanotubes in plant systems are able to change metabolic acts that lead to increase products . XRD and nanoparticle sizer experiments showed that the major phase is carbon and its size is range of 35-300 nm. The aim of this study is to investigate the effect of concentrations of multi-walled carbon nanotubes (MWCNTs) (0 and 500 µg ml-1 ) alongside the control under laboratory conditions on the characters of histology and ultrastructure. The vegetative growth was done in greenhouse conditions .foliar spray 500 µg ml-1 treatment (MWCNTs and deionezed water for 0 treatment) was done. Duration of experiment was 45 days. after that plant was exited from soil, shoot and root were seprated.process of preparation was done and samples go over on grid for study TEM.The result of ultra strcture studies of root and leaf showed, there is great aggregation of multi-walled carbon nanotubes with different length in vacuole and cytoplasm which in leaf cells have aggregation of vacuole and increase of plastogloboly in stroma of chlroplast and transformation of chloroplast from globular to ellipsoid and in root cells lead to destruction of mitochondria crista, vacoule and nucleus membrane and low chromatins, also was observed aggregation of vacuole and many diameter of sclreid and xylem wall.

References:

[1]     Çaglayan, K., Serce, C., Barutcu, E., Kaya, K., Medina, V., Gazel, M., Soylu, S. and Caliskan, O. S.; Vadan R.; Dai L.; Luo M.; Ambikapathi R.; Lee E. H. And Olszyk D. 2008, Comparison by Sequence-Based and Electron Microscopic Analyses of Fig Mosaic Virus Isolates Obtained from Field and Experimentally Inoculated Fig Plants. Plant Disease, 94, 1448-1452.

[2]     Casey, A., Farrell, G.F., McNamara, M., Byrne, H.J. and Chambers, G. 2005, Interaction of Carbon Nanotubes with Sugar Complexes. Synthetic Metals, 153, 357-360.

[3]     Flores, D.; Chacón, R.; Alvarado, L.; Schmidt, A.; Alvarado, C. and Chaves, J. 2014, Effect of Using Two Different Types of Carbon Nanotubes for Blackberry (Rubus adenotrichos) in Vitro Plant Rooting, Growth and Histology, American Journal of Plant Sciences, 5, 3510-3518.

[4]     Gardea-Torresdey, J. L., Gomez, E., Peralta-Videa, J. R., Parsons, J. G., Troiani, H., and Jose-Yacaman, M. 2003, Alfalfa sprouts: a natural source for the synthesis of silver nanoparticles. Langmuir, 19(4), 1357-1361.

[5]     Hofinger H.; Melle-Franco M.; Gallo T.; Cantelli A.; Calvaresi M., Gomes J. A. N. F. And Zerbetto F. 2011, A Computational analysis of the insertion of carbon nanotubes in to cellular membranes, Biomaterials, 32: 7079- 7085.

[6]     Husen A. and  Siddiqi K. 2014, Carbon and fullerene nanomaterials in plant system. J Nanobiotechnol;12:16.

[7]     Kamil Ahmed M., Hussein Falah H., Halbus Ahmed F. and Bahnemann Detlef W. 2014 Preparation, Characterization and Photocatalytic Applications of MWCNTs/TiO₂ Composite, International Journal of Photoenergy, Volum 2014, ID 475713, 8page.

[8]     Khan, A. and Sanwal, M. 2015, Medicinal Importance of Ricinus communis (Arandi), International Journal of Current Trends in Pharmaceutical Research, 3(3): 868-941.

[9]     Khodakovskaya, M.; Dervishi, E.; Mahmood, M.; Xu, Y.; Li, Z.; Watanabe, F. and Biris, A. S. 2009, Carbon Nanotubes Are Able To Penetrate Plant Seed Coat and Dramatically Affect Seed Germination and Plant Growth, American Chemical Society, ARTICLE, 3 (10): 3221–3227, review.

[10] Khodakovskaya, M. V.; de Silva, K.; Biris, A. S.; Dervishi, E.and Villagarcia, H. 2012, Carbon Nanotubes Induce Growth Enhancement of Tobacco Cells, ACS Nano, 6 (3), pp 2128–2135.

[11] Lahiani,M. H.; Dervishi, E.; Chen, J.; Nima, Z.; Gaume, A.; Biris, A. S. and Khodakovskaya, M. V. 2013, Impact of Carbon Nanotube Exposure to Seeds of Valuable Crops, American Chemical Society, 5 (16): 7965–7973.

[12] Ma, X.; Geiser-Lee, J.; Deng, Y.; Kolmakov, A. 2010. Interactions between engineered nanoparticles (ENPs) and plants: Phytotoxicity, uptake and accumulation. Sci. Total Environ.,408: 3053–3061.

[13] Mani, U. ; Dhanasingh,S.; Arunachalam, R.; Paul, E.; Shanmugam, P.; Rose, C.; Baran Mandal, A. 2013, A Simple and Green Method for the Synthesis of Silver Nanoparticles Using  Ricinus Communis Leaf Extract, Progress in Nanotechnology and Nanomaterials, 2 ( 1): 21-25.

[14] Martínez-Ballest M C.; Zapata L.; Chalbi N. and Carvajal M. 2016, Multiwalled carbon nanotubes enter broccoli cells enhancing growth and water uptake of plants exposed to salinity, Journal of Nanobiotechnology,14: 42.

[15] Milne, W.I.; Teo, K.B.K.; Amaratunga, G.A.J.; Legagneux, P.; Gangloff, L.; Schnell, J.P.; Semet, V.; Binh, V.T.; Groening, O. 2004. Carbon nanotubes as field emission sources. J. Mater. Chem, 14, 933–943.

[16] Nel, A.; Xia, T.; Meng, H.; Wang, X.; Lin, S.; Ji, Z.; Zhang, H. 2013, Nanomaterial toxicity testing in the 21st century: Use of a predictive toxicological approach and high-throughput screening. Acc.Chem. Res., 46: 607–621.

[17] Pourkhaloee, A.; Haghighi,M.; Saharkhiz, M. J.; Jouzi, H.and Doroodmand, M. M. 2011, Carbon nanotubes can promote seed germination via seed coat penetration. Journal of Seed Technology. 33 (2): 155-169.

[18] Pogodin S. And Baulin V. A. 2010, Can a carbon nanotube pierce through a phospholipid bilayer? , 1010-1462.

[19] Prakash, E.and Gupta, D.K. 2014, In Vitro Study of Extracts of Ricinus communis Linn on Human Cancer Cell lines,  Journal of Medical Sciences and Public Health, 2 (1): 15-20.

[20] Ray, M. 2010, The Effects of Single-Walled Carbon Nanotubes on Plant Growth, Electronic Theses and Dissertations.Paper 90.

[21] Srinivasan, C.; Saraswathi, R. 2010, Nanoagriculture—carbon nanotubes enhance  tomato seed germination and plant growth. Curr Sci 99:274–275.

[22] Tiwari, D. K.; Dasgupta-Schubert, N.; Villasen˜or Cendejas, L. M.; Villegas, J.; Carreto Montoya, L.; Borjas Garcı´a., S. E. 2014, Interfacing carbon nanotubes (CNT) with plants:enhancement of growth, water and ionic nutrient uptake in maize (Zea mays) and implications for nanoagriculture, Appl Nanosci, 4:577–591.

[23] Yuan H.; Hu S. H.; Huang P.; Song H.; Wang K.; Rruan J.; He R. And Cui D. 2011, Single walled carbon nanotubes exhibit dual-phase regulation to exposed Arabidopsis mesophyll cells. Nanoscale Research Letters, 6: 44-52.