Study of TEM and SHV B-lactamase genes in gentamicin resistant Enterococcus faecalis isolated from consumption red meats.

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Rezvan Shirvani ¹ ( Borujerd Azad university )
Mohammadreza Mehrabi ² ( Islamic Azad University )
Mohsen Mirzaee ³ ( دانشگاه آزاد اسلامی واحد بروجرد )

Received: 2017-01-24 Accepted : 2017-02-12 Published : 2017-02-19

Keywords: polymerase chain reaction, Enterococcus faecalis, Gentamicins,

Abstract :

Background and Objectives: Enterococci are part of normal flora of the human gastrointestinal tract. The ability to obtain antibiotic resistance genes, causes new problems in treatment. Enterococcus faecalis is one of the bacteria contaminating the meat and cause significant infections in humans. So the purpose of this study was evaluation prevalence of bla TEM and bla SHV genes in gentamicin resistant Enterococcus faecalis isolated from consumption meats. Materials and methods: 181 Enterococcus isolates were collected from consumption meats in Boroujerd slaughterhouse. Bacteria were identified by routine microbiological tests. The antibiotic susceptibility testing on isolates by disk diffusion method according to the CLSI was performed. Finally, to assess the frequency of bla TEM and bla SHV genes in gentamicin resistant Enterococcus faecalis isolated from consumption meats were performed by using PCR and specific primers. Results: Out of the 181 isolates 81 cases (44.75%) were E. faecalis. The results of the antibiogram test, showed that 96.29% of bacteria were resistance to erythromycin, 56.79% to penicillin, 41.96% to tetracycline, 39.50% to ampicillin,39.50% to choloroamfenicol,15.86% to Linezolid, 8.64% to Gentamicins, 4.83% to Streptomycin, 2.46% to Meropenem,3.70% to ciprofloxacin and in the strains gentamicin resistant bla TEM and bla SHV genes not found. Conclusion: Given that this fact the genes producing Betalactamase enzymes are easily transferred via plasmids, lack of tracking the mentioned genes among the studied bacteria suggests, these genes are not transferred along with genes inducing resistance to gentamicin. thus rational and significant relationship between these genes and investigated antibiotic was not observed.

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