Comparison of preservative effect of effective material of ecotypes of Tracheyspermum ammi on the growth of citrus fungi.
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
atefeh
habibi roodbari
1
(Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran)
mohaddeseh
larypoor
2
(Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran)
Fariba
Sharifnia
3
(Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran)
Keywords: Ecotype, Essence, Alternaria alternata, Trachyspermum ammi fruit, Citrus preservative,
Abstract :
Introduction and aims: In this study , we reviewed the comparison the chemical content of active ingredient was isolated from Trachyspermum ammi Plant of three bioclimatic zones of Iran as preserver of Citrous fruits on the growth of Alternaria alternata, Penicillium digitatum, Aspergillus niger .Methods: essential oil of Trachyspermum ammi fruit from different ecotypes were prepared by hydrodistillation in a Clevenger-type apparatus and were quantified and identified by comparison with standard spectra after GC-MS analysis. Antifungal effect of essential oils was evaluated by disk diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC) and 50% inhibition of fungal growth (IC50). The results: All essential oil ecotypes in the disk fusion method had a growth inhibition zone greater than 10 cm but we saw the growth of fungi with extracts and sweets of essential oil. The most effective ecotype of essential oils on the studied fungi were the Shiraz and Kerman ecotype for Aspergillus niger with MIC and MFC equal 125 and 250 ug/ml. The lowest percentage of growth inhibition is realated to essential oil of Shiraz for Alternaria alternata and Esfahan for Aspergillus niger in concentration 31/25 to 2000 ug/ml between (5/58-100%)-(5/3-100%). Discussion: The finding of this study indicates that essential oil compared to extract and sweat of this plant has a significant antifungal effect on the fungi studied. So you can hope that in the future, replacing herbal essential oil instead of chemical antifungal drugs that always have a lot of side effects can used as citrus retainers.
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