اثر BAP و NAA روی ریزازدیادی کالادیوم Caladium bicolor (Aiton) Vent.))، یک گیاه زینتی

Subject Areas : Journal of Ornamental Plants

شیما صیدی ¹ , ناصر نگهدار ² , راضیه تقی زاده اندواری ³ , محمد حسین انصاری ⁴ , بهزاد کاویانی ⁵

1 - Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran
2 - Master of Science and Ph.D. Student, Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran
3 - Master of Science, Hyrcan Agricultural Sciences and Biotechnology Research Institute, Amol, Iran
4 - Department of Agronomy, Rasht Branch, Islamic Azad University, Rasht, Iran
5 - Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran

Received: 2015-10-08 Accepted : 2016-01-15 Published : 2016-03-01

Keywords: کشت بافت, آراسه, تکثیر درون شیشه‌ای, تنظیم کننده‌های رشد گیاهی,

Abstract :

ریزنمونه‌های برگ کالادیوم در شرایط درون شیشه‌ای روی محیط‌های MS همراه با 25 غلظت مختلف از BAP و NAA به‌منظور تعیین غلظت‌های مناسب برای ریزازدیادی کشت شدند. تمام ترکیبات تشکیل کالوس را روی ریز نمونه‌ها القا کردند. تولید کالوس روی ریزنمونه‌های برگی رشد یافته روی محیط شاهد بسیار کم بود. محیط غنی شده با 4 میلی‌گرم بر لیتر BAP + 5/0 میلی‌گرم بر لیتر NAA برای تشکیل کالوس موثرترین بودند. بیشترین تعداد نوشاخه‌ها (43/6 در ریزنمونه) و بیشترین تعداد ریشه‌ها (56/5 در ریز نمونه) به‌ترتیب روی محیط‌های حاوی یک میلی‌گرم بر لیتر BAP + 5/0 میلی‌گرم بر لیتر NAA و 3 میلی‌گرم بر لیتر BAP + 5/0 میلی‌گرم بر لیتر NAA تولید شدند. گیاهچه‌های باززایی شده در شرایط گلخانه‌ای رشد داده شدند و بطور موفقیت‌آمیزی سازگار گردیدند.

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