اثرات وابسته به دوز سریا (CeO2) با اندازه نانو بر روی جوانه زنی بذر، رشد اولیه و پارامترهای فیزیولوژیکی گیاهچه همیشه بهار

Subject Areas : Journal of Ornamental Plants

صدیقه  جهانی ¹ (گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)
سارا  سعادتمند ² (گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)
ملیحه  جهانی ³ (گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)
هما  محمودزاده ⁴ (گروه زیست شناسی، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران)
رمضانعلی  خاوری نژاد ⁵ (گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران)

Received: 2022-03-19 Accepted : 2022-05-22 Published : 2022-06-01

Keywords: درصد و سرعت جوانه‌زنی, نانوسمیت, سیستم دفاعی گیاه, طول ریشه‌چه و ساقه‌چه, .Calendula officinalis L,

Abstract :

همیشه بهار به عنوان یک گیاه زینتی-دارویی کاربرد فراوانی دارد. برهمکنش بین نانوذرات و سیستم‌های بیولوژیکی یکی از امیدوارکننده‌ترین حوزه‌های تحقیقاتی در علم و فناوری نانومدرن است. محققان جذب نانوذرات اکسید سریم یا سریا (CeO2) توسط گیاهان را گزارش کرده‌اند. هدف از این تحقیق بررسی تاثیر نانوسریا بر جوانه‌زنی بذر، ویژگی‌های رشد و بیوشیمیایی گیاهچه 9 روزه همیشه بهار (.Calendula officinalis L) بود. بذرها در پتری‌دیش‌های حاوی هشت غلظت مختلف نانوسریا (0، 0.05، 0.1، 0.2، 0.4، 0.8، 1.6 و 3.2 گرم بر لیتر) جوانه زدند. پس از 9 روز، پارامترهای رشد اولیه و بیوشیمیایی اندازه‌گیری شد. نتایج نشان داد که جوانه‌زنی بذر، وزن تر و خشک گیاهچه و طول ریشه‌چه، ساقه‌چه و گیاهچه در غلظت‌های 0.05 و/ یا 0.1 گرم بر لیتر نانوسریا تحریک شد اما در غلظت‌های بالاتر (پس از 0.2 یا 0.4 گرم بر لیتر) نانوذرات به تاخیر افتاد. محتوای H2O2، مالون‌دی‌آلدئید (MDA) و فعالیت لیپواکسیژناز (LOX) پس از 0.2 گرم بر لیتر نانوسریا افزایش یافت. فعالیت آنزیم‌های آنتی‌اکسیدان و محتوای پروتئین در غلظت‌های بالاتر نانوسریا افزایش یافت. همچنین، فعالیت آنزیم فنیل آلانین آمونیالیاز (PAL)، محتوای فنل و ظرفیت آنتی‌اکسیدانی در 0.8 تا 3.2 گرم بر لیتر نانوسریا تحریک شد. محتوای پرولین در 0.2-3.2 گرم بر لیتر نانوسریا بهبود یافت. در مجموع، نتایج نشان داد که تنش اکسیداتیو القایی نانوسریا با سیستم دفاعی گیاه شامل آنزیم‌های آنتی‌اکسیدان، ترکیبات فنلی و اسمولیت‌های سازگار مانند پرولین همراه بود. این نتایج نشان داد که نانوسریا در غلظت‌های پایین (0.05 و/ یا 0.1 گرم بر لیتر) باعث القای مثبت بر روی جوانه‌زنی بذر و رشد گیاهچه همیشه بهار شد، اما با افزایش غلظت آن (بیشتر از 0.2 گرم بر لیتر)، نتیجه برعکس شد و سمیت نشان داد که گیاه را مجبور کرد تا سیستم‌های دفاعی خود را فعال کند.

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