تاثیر پاتوتیپ برگریز Verticillium dahliae بر برخی از ویژگی‌های فیزیولوژیکی و بیوشیمیایی داوودی (Dendranthema grandiflorum (Ramat.) Kitam)

Subject Areas : Journal of Ornamental Plants

seyed Javad Sanei ¹ ( Plant protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran )
Seyed Esmael Razavi ² ( Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran )

Received: 2017-07-04 Accepted : 2017-11-26 Published : 2018-03-03

Keywords: پژمردگی, آنزیم‌های آنتی‌اکسیدانت, گونه‌های فعال اکسیژن, &lt, i&gt, Verticillium dahliae&lt, /i&gt, ,

Abstract :

Verticillium dahliae Kleb. از قارچ­های بیمارگر خاک­زی است که عامل پژمردگی ورتیسیلیومی گیاه داوودی می­باشد. در این تحقیق، سطوح برخی از ترکیبات آلی، ازجمله پروتئین کل، قندهای محلول کل، نشاسته، پرولین، محتوای نسبی آب، سطح RNA و مقدار مالون دی­آلدهید و H2O2 در برگ­های داوودی آلوده به V. dahliae بررسی شد. تغییر پارامترها در روزهای 0، 10، 20، 30 و 40 روز پس از تلقیح با روش­های مبتنی بر رنگ­سنجی اندازه­گیری شد. بر اساس نتایج، قبل از ظهور علائم تغییری در محتوای نسبی آب، RNA و پروتئین مشاهده نشد و تغییر در میزان کلروفیل اندک بود. تغییر در محتوای نسبی آب همراه با تغییرات قابل­توجه در پرولین و قندهای محلول کل برگ همراه بود. مقدار نشاسته و پروتئین به­تدریج در برگ­های سالم و آلوده کاهش داشت. اگرچه، این کاهش در گیاهان آلوده از هفته سوم پس از آلودگی قابل­توجه بود. همبستگی منفی بالایی بین قندهای محلول کل و نشاسته در برگ­های بیمار مشاهده شد (001/0P<، 764/0=r). تغییرات مالون دی­آلدهید و H2O2 در گیاهان آلوده 30 الی 40 روز پس از آلودگی رخ داد، در حالی که غلظت آن­ها در برگ­های شاهد تغییری نداشت. این نتایج نشانگر نقش پیری همراه با پیشرفت بیماری پژمردگی ورتیسیلیومی در داوودی است.

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