Survey of the Protoscoli Effect of Hydatid Cyst on Production of Cysts in Rabbit

Subject Areas : Large Animal Diseases

Said Hashemi ¹ , Liela Derkhshan ² , Kimia Nejati ³ , Melina Deghighian ⁴

1 - Department of Animal Sciences, Faculty of Agriculture, Boroujerd Azad University, Boroujerd, Iran
2 - Department of Pathobiology, Faculty of Veterinary Medicine, Shushtar Azad University, Shushtar, Iran
3 - Graduated from the Faculty of Veterinary Medicine, Shushtar Azad University, Shushtar, Iran
4 - Student from the Faculty of Veterinary Medicine, Shushtar Azad University, Shushtar, Iran

Received: 2020-10-08 Accepted : 2021-01-11 Published : 2021-01-20

Keywords: hydatid cyst fluid, Protoscolex, Ankoura Rabbit,

Abstract :

In order to investigate the effect of protoscolex hydatid cyst on the production of cysts in rabbits, from May 1401 to Tanime Khordad, a total of 25 sheep hydatid cyst samples, including 10 lung cyst samples and 15 liver cyst samples, were collected from Durood slaughterhouse and 3 ml of cyst fluid were collected under sterile conditions. aspirated and after centrifugation at 1500 rpm for 5 minutes, we poured the protoscolexes into a 2 ml Eppendorf tube. The viability of protoscolex was determined with 1% eosin dye with a light microscope. 2 groups of 4 female (group one) and male (group two) rabbits were tested. One sample from each group was not prescribed any drug or protoscolex as a control. Three rabbits from one group were injected intraperitoneally with one milliliter of cyst fluid containing about 2000 live protoscolex. The same amount of lung cyst was administered to three others from group two. After 45 days, the rabbits were killed with ether and autopsied, and cysts were isolated in two cases on the liver and peritoneal area. The size of the cyst in one female rabbit included a liver cyst of 12 mm in length and 7.8 mm in width, and in another rabbit, an abdominal cyst of 8.5 mm in length and 2.7 in width was seen. but they lacked liquid and protoscolex, so it seems that the protoscolex hydatid cyst of sheep has the ability to produce cysts in laboratory animals.