The Effects of Azathioprine on Kidny Tissue in Dibetic Rats

Subject Areas : Large Animal Diseases

Farangis Ghasemy ¹ (1. Student of PhD Degree in Veterinary Anatomy, Teaching staff of Biology Group, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Abbas Hushmandy ² (2. Master Degree in Animal physiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran)
Golshid Ahmady ³ (3. Faculty of Dentistry, Shiraz Medical University, International Branch, Shiraz, Iran)

Received: 2015-12-16 Accepted : 2015-12-16 Published : 2013-09-23

Keywords: Azathioprine, tissue, Collecting tubes, Glomerols,

Abstract :

Diabetes is a metabolic disease that results in major damage to the kidney. Azathioprine is widely used in the treatment of other autoimmune diseases, cancer, and organ transplantation. In this study, the effect of azathioprine on renal tissue and serum markers of insulin resistance in rats were studied. 56 adult male wistar mouse with 80 to 90 days old and weighing approximately 200±20g were diveded in 8 groups as follow: Control group (without treatment), sham (140cc froctose 10% as a daily feeding) and 1 to 4 experimental groups that in addition of 140cc froctose 10% as a daily feeding, were injected [3.75, 7.5, 15 and 21] mg/kg/bw azathioprine both in single dose and interaperitoneal at 96th day. 24 hours after both kidneys were seprated, fixed in formalin (10%) and placed in tissue processor. Prepared serial sections were studied with LM (10x & 40x). Obtain data analyzed by using SPSS (17) and ANOVA and groups were compared in significance level (P< 0.05) with Tukey's test. Significant decrease in the diameter of the renal cortex and medulla in control, diabetic and non-diabetic treatment groups and a significant increase in proximal and distal convouluted, henle's loop and glomerules compared with the control group. Collecting tubes  difference was not significant in all groups compared to controls. According to above results, Azathioprine creating synergistic effects with diabete. Therefore, diabetics are taking limits.

References:

1-Ahmad, MS, Ahmed, N, 2006. Antiglycation properties of aged garlic extract

    possible role in prevention of diabetic complications. Journal of  Nutrition, 796-799.

2-Amin, A, Hamza, AA, 2005 . Hepatoprotective effects of Hibiscus, Rosmarinus 

   and Salvia on azathioprine induced toxicity in rats. Life Science, 77(3): 266-78.

3-Amouoghli Tabrizi, B, Mohajeri, D, Doostar, Y, Baradaran Alizade, et al,. 2009. 

   Biochemical and pathological study of protective effect of Vitamin A in Azathioprine- 

   induced hepatotoxicity in Rat, Feyz, Jouranal of Kashan University of Medical

   Sciences. 13: 180-187.

4-Barnett AH, Bain SC, Bouter P, Karlberg B, Madsbad S, Jervell J, Mustonen J 2004.

    Diabetics Exposed to Telmisartan and Enalapril Study Group: Angiotensin-receptor

   blockade versus converting-enzyme inhibition in type 2 diabetes and nephropathy. N 

   Engl J Med 351: 1952 –1961. 

5- David, M, 1993. Long-term complications of diabetes mellitus.New England Journal 

   of Medicine, 328:1676-1685.

6- Hayashi, T, Nozawa, M, Sohmiya, K, Toko, H, Nakao, M, et al,. 1998. Efficacy of 

   pancreatic transplantation on cardiovascular alterations in diabetic rats: an ltrastructural 

   and immunohistochemical study. Transplant Protocol, 30: 335-8.

7-Kaneto, H, Katakami, N, Kawamori, D, et al,. 2007. Involvement of oxidative stress in 

   the pathogenesis of diabetes. Antioxidants & Redox Signaling, 9 (3): 355-66.

8-Rainer, L, Schleicher, ED, 2000. Molecular mechanism of diabetic nephropathy. 

   Clinica Chimical Acta, 297:135–44.

9- Remuzzi G, Ruggenenti P, Perna A, Dimitrov BD, de Zeeuw D, Hille DA,et al. 2004.

   Continuum of renoprotection with losartan at all stages of type 2 diabetic nephropathy: 

   A post hoc analysis of the RENAAL trial results. J Am Soc Nephrol 15 :3117 –3125.

10-Sundby, GB, Ormstad, K, Orrenius, S, 1979. Use of isolated kidney cells for study of 

   drug metabolism. Biochemical Pharmacology, 28:929–935. 

11-Tapner, M, Jones, B, Farrel, G, 2004. Toxicity of low dose azathioprine and 6-

   mercaptopurine in rat hepatocytes. Roles of xanthine oxidase and mitochondrial injury,

   Journal of Hepatology, 40(3): 454-463.

12-Tripathi BK, and Srivastava AK (2006) Diabetes mellitus:complications and 

   herapeutics. Med Sci Monit, , 12: 130-147.

13-Wu, YG, Lin, H, Qi, XM, Wu, GZ, et al,. 2006. Prevention of early renal injury by 

   mycophenolate mofetil and its mechanism in experimental diabetes. International 

   Immunopharmacology, 445–53.