بررسی اثر متقابل فعالیت دو آنزیم کبدی و برخی مواد معدنی موجود در کبد جوجههای گوشتی
Subject Areas : Camel
1 - Department of Animal Science, FacultyofAgricultur, Shahrekord University, Shahrekord, Iran
Keywords: IDH, MDH, جوجه گوشتی, مواد معدنی, اثر متقابل آنزیم,
Abstract :
هدف از انجام این پژوهش بررسی اثر متقابل فعالیت دو آنزیم کبدی و برخی مواد معدنی موجود در کبد جوجههای گوشتی بوده است. تحقیق بر روی جوجههای گوشتی نر و ماده از سن 1 تا 56 روزگی انجام شد. اسید مالیک از سن 1 روزگی به آب آشامیدنی جوجه‌ها در غلظت‌های صفر (شاهد)، 05/0، 10/0 و 15/0 درصد اضافه شد و به طور آزاد در اختیار آنها قرار گرفت. جوجه‌ها در سن 56 روزگی کشتار شده و بلافاصله کبد آنها جدا و قسمتی از آن هموژنایز و عصاره‌گیری شد و در عصارة حاصل، فعالیت آنزیم‌های میلیت دهیدروژناز (MDH) و آیزوسیتریت دهیدروژناز (IDH) اندازه‌گیری شد. مواد معدنی نیز در باقیمانده کبد که در آون خشک شده بود، اندازه‌گیری شد. نتایج نشان داد که فعالیت IDH با افزایش غلظت میلیت مصرفی افزایش می‌یابد (05/0P<) ولی فعالیت MDH تفاوت معنی‌داری را بین تیمارهای تحت آزمون نشان نداد (05/0P<). جوجههای نر نیز 28 درصد بیشتر از جوجههای ماده فعالیت آنزیم IDH نشان دادند (05/0P<). روی و آهن نیز به ترتیب همبستگی معنی‌داری با فعالیت IDH و MDH نشان دادند (05/0P<). هیچ نوع همبستگی منفی و معنی‌داری بین غلظت مواد معدنی و غلظت سلنیوم کبد مشاهده نشد (05/0P<). البته همبستگی مثبت و معنی‌دار بین غلظت سرب، منیزیوم، نیکل، منگنز، جیوه و کبالت در کبد جوجه‌ها مشاهده شد. نتیجه اینکه، افزودن میلیت به آب آشامیدنی جوجه‌ها سبب شد تا غلظت آهن در کبد افزایش یابد اما روی ذخیره‌سازی سایر مواد معدنی تأثیر معنی‌داری مشاهده نگردید.
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