نقش هورمون FSH در بقا، فعال‌سازی و رشد بیشترکشت آزمایشگاهی فولیکول‌های بدوی گوسفند

Subject Areas : Camel

ف. اسماعیل زاده ¹ , س. م. حسینی ² , م. حاجیان ³ , ز. نصیری ⁴ , م. چمنی ⁵ , م. امین افشار ⁶ , M.H. Nasr-Esfahani ⁷

1 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
3 - Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
4 - Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran
5 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
6 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
7 - Department of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran

Received: 2012-05-01 Accepted : 2012-09-27 Published : 2013-12-01

Keywords: FSH, فولیکول‌های بدوی گوسفند, کشت آزمایشگاهی, فعال‌سازی,

Abstract :

  هدف از این مطالعه بررسی اثر هورمون FSH بر بقا، فعال‌سازی و رشد بیشتر کشت آزمایشگاهی فولیکول‌های بدوی گوسفند با استفاده از مطالعات بافت‌شناسی است. قطعات قشری بافت تخمدان در دو گروه کنترل کشت‌شده (محیط کشت MEM) و آزمایشی (محیط کشت پایه+هورمون FSH (غلظت ثابت 50ng/mL )) به مدت شش روز کشت گردید. سپس قطعات کشت ‌نشده و کشت ‌شده برای ارزیابی مورفولوژیکی (در سه گروه) فرآوری شدند. همچنین اثر هورمون FSH بر قطر فولیکول‌ها و اووسیت‌های کشت ‌شده بررسی گردید. نتایج نشان داد که پس از شش روز کشت، کمترین و بیشترین درصد فولیکول‌های نرمال و رشد کرده به ترتیب در گروه‌های کنترل کشت ‌شده و آزمایشی به دست آمد. همچنین بیشترین افزایش قطر اووسیت و فولیکول‌های نرمال در گروه آزمایشی با غلظت ثابت هورمون FSH (50ng/mL ) ملاحظه شد. بنابراین عامل FSH در روند تکوین، تداوم رشد و بقای فولیکول‌های بدوی تخمدان گوسفند نقش مؤثری دارد.

References:

Adriaens I., Cortvrindt R. and Smitz J. (2004). Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence. Hum. Reprod. 19, 398-408.
Andrade E.R., Maddox-Hyttel P., Da Cruz Landim-Alvarenga F., Silva J.R.V., Alfieri A.A., Seneda M.M., Figueiredo J.R. and Toniolli R. (2011). Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic Acid, EGF, and FSH. Vet. Med. Int. 7(2), 80-89.
Andrade E.R., Seneda M.M., Alfieri A.A., Oliveira J.A., Bracarense A.P.F.R.L., Figueiredo J.R. and Toniolli R. (2005). Interactions of indole acetic acid with EGF and FSH in the culture of ovine preantral follicles. Theriogenology. 64, 1104-1113.
Chun S.Y., Billig H., Tilly J.L., Furuta I., Tsafriri A. and Hsueh A.J. (1994). Gonadotropin suppression of apoptosis in cultured preovulatory follicles: mediatory role of endogenous insulin-like growth factor I. Endocrinology. 135, 1845-1853.
Cortvrindt R., Smitz J. and Van Steirteghem A.C. (1997). Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in vitro. Hum. Reprod. 12, 759-768.
Dierich A., Sairam M.R., Monaco L., Fimia G.M., Gansmuller A., LeMeur M. and Sassone-Corsi P. (1998). Impairing follicle-stimulating hormone (FSH) signaling in vivo: targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance. Proc. Natl. Acad. Sci. USA. 95, 13612-13617.
Durlinger A.L., Gruijters M.J., Kramer P., Karels B., Ingraham H.A., Nachtigal M.W., Uilenbroek J.T., Grootegoed J.A. and Themmen A.P. (2002). Anti-mullerian hormone inhibits initiation of primordial follicle growth in the mouse ovary. Endocrinology. 143, 1076-1084.
Hsueh A.J., Billig H. and Tsafriri A. (1994). Ovarian follicle atresia: a hormonally controlled apoptotic process. Endocrinol. Rev. 15, 707-724.
Itoh T., Kacchi M., Abe H., Sendai Y. and Hoshi H. (2002). Growth, antrum formation, and estradiol production of bovine preantral follicles cultured in a serum-free medium. Biol. Reprod. 67, 1099-1105.
Lima-Verde I.B., Rossetto R., Matos M.H.T., Celestino J.J.H., Bruno J.B., Silva C.M.G., Faustino L.R., Mororó M.B.S., Araújo V.R., Campello C.C. and Figueiredo J.R. (2010). Androstenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro. Anim. Reprod. 7(2), 80-89.
Magalhães D.M., Araújo V.R., Lima-Verde I.B., Matos M.H.T., Silva R.C., Lucci C.M., Báo S.N., Campello C.C. and Figueiredo J.R. (2009). Impact of pituitary FSH purification on in vitro early folliculogenesis in goats. Biocell. 33, 91-97.
Mao J., Wu G., Smith M.F., McCauley T.C., Cantley T.C., Prather R.S., Didion B.A. and Day B.N. (2002). Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro. Biol. Reprod. 67, 1197-1203.
Matos M.H., Lima-Verde I.B., Bruno J.B., Lopes C.A., Martins F.S., Santos K.D., Rocha R.M., Silva J.R., Báo S.N. and Figueiredo J.R. (2007a). Follicle stimulating hormone and fibroblast growth factor-2 interact and promote goat primordial follicle development in vitro. Reprod. Fertil. Dev. 19(5), 677-684.
Matos M.H., Lima-Verde I.B., Luque M.C., Maia J.E., Silva J.R., Celestino J.J., Martins F.S., Báo S.N., Lucci C.M. and Figueiredo J.R. (2007b). Essential role of follicle stimulating hormone in the maintenance of caprine preantral follicle viability in vitro. Zygote. 15(2), 173-182.
McGee E., Spears N., Minami S., Hsu S.Y., Chun S.Y., Billig H. and Hsueh A.J.W. (1997). Preantral ovarian follicles in serum-free culture: suppression of apoptosis after activation of the cyclic guanosine 3’-5’-monophosphate pathway and stimulation of growth and differentiation by follicle-stimulating hormone. Endocrinology. 138, 2417-2424.
Méduri G., Charnaux N., Driancourt M.A., Combettes L., Granet P., Vannier B., Loosfelt H. and Milgrom E. (2002). Follicle-stimulating hormone receptors in oocytes? J. Clin. Endocrinol. Metab. 87, 2266-2276.
O’Brien M.J., Pendola J.K. and Eppig J.J. (2003). A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence. Biol. Reprod. 68, 1682-1686.
Oktay K., Briggs D. and Gosden R.G. (1997). Ontogeny of follicle-stimulating hormone receptor gene expression in solated human ovarian follicles. J. Clin. Endocrinol. Metab. 82, 3748-3751.
Oktay K., Newton H., Mullan J. and Gosden R.G. (1998). Development of human primordial follicles to antral stages in SCID/hpg mice stimulated with follicle stimulating hormone. Hum. Reprod. 13, 1133-1138.
O'Shaughnessy P.J., Dudley K. and Rajapaksha W.R. (1996). Expression of follicle stimulating hormone-receptor mRNA during gonadal development. Mol. Cell. Endocrinol. 125, 169-175.
Peng X., Yang M., Wang L., Tong C. and Guo Z. (2010). In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium. J. Assist. Reprod. Genet. 101, 30-41.
Picton H.M., Harris S.E., Muruvi W. and Chambers E.L. (2008). The in vitro growth and maturation of follicles. Reproduction. 136, 703-715.
Saha S., Shimizu M., Geshi M. and Izaike Y. (2000). In vitro culture of bovine preantral follicles. Anim. Reprod. Sci. 63, 27-39.
Silva J.R.V., Van den Hurk R., Matos M.H.T., Santos R.R., Pessoa C., Moraed M.O. and Figueiredo J.R. (2004). Influences of FSH and EGF on primordial follicles during in vitro culture of caprine ovarian cortical tissue. Theriogenology. 61, 1691-1704.
Thomas F.H., Ethier J.F., Shimasaki S. and Vanderhyden B.C. (2005). Follicle-stimulating hormone regulates oocyte growth by modulation of expression of oocyte and granulosa cell factors. Endocrinology. 146, 941-949.
Touraine P., Beau I., Gougeon A., Meduri G., Desroches A., Pichard C., Detoeuf M., Paniel B., Prieur M., Zorn J.R., Milgrom E., Kutten F. and Misrahi M. (1999). New natural inactivating mutations of the follicle-stimulating hormone receptor: correlations between receptor function and phenotype. Mol. Endocrinol. 13, 1844-1854.
Van den Hurk R. and Zhao J. (2005). Formation of mammalian oocytes and their growth, differentiation and maturation within ovarian follicles. Theriogenology. 63, 1717-1751.
Yu-bin L., Can-quan Z., Guo-fen Y., Qiong W. and Yu D. (2007). Modified vitrification method for cryopreservation of human ovarian tissues. Chin. Med. J. 120(2), 110-114.
Zhou H. and Zhang Y. (2005). Regulation of in vitro growth of preantral follicles by growth factors in goats. Domest. Anim. Endocrinol. 28, 235-242.