اثر چندشکلیهای UCP روی صفات رشد، ارزش اصلاحی صفات رشد و صفات تولیدمثلی در مرغ بومی فارس
Subject Areas : Camelآ. محمدی‌فر 1 , م.ر. محمدآبادی 2
1 - Department of Animal Science, Faculty of Agriculture, Payame Noor University, Kerman, Iran
2 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
Keywords: PCR-RFLP, چندشکلی, صفات تولیدمثلی, مرغ بومی فارس, پروتئین غیر مزدوج,
Abstract :
پروتئین غیر مزدوج طیور (avUCP) عضوی از فوق خانواده ناقلین میتوکندریایی است که ورود پروتئین به ماتریکس میتوکندری را از سنتز ATP آزاد میکند. از روش PCR-RFLP برای برآورد فراوانیهای آللی و ژنوتیپی چندشکلیهای UCP/HhaI استفاده شد و همبستگی بین این چندشکلیها و صفات رشد، ارزش اصلاحی صفات رشد و تولیدمثلی در مرغ بومی فارس محاسبه شد. برای این منظور اطلاعات فنوتیپی 18 نسل متوالی از 200 پرنده با استفاده از مدل حیوانی تک صفتی با روش ASREML آنالیز شد. ارزیابی همبستگی بین این SNP با صفات تولیدمثلی اثر مثبت ژنوتیپ TC را با سن اولین تخمگذاری (ASM) در مقایسه با ژنوتیپ CC پیشنهاد میکند. به علاوه، ژنوتیپ TC همبستگی معنی‌داری با ارزش اصلاحی سن اولین تخمگذاری در مقایسه با ژنوتیپ CC نشان داد (05/0>P). در نتیجه، نتایج این پژوهش پیشنهاد میکند که ژنوتیپ TC ژن UCP با سن بلوغ جنسی (ASM) و ارزش اصلاحی سن بلوغ جنسی همبستگی دارد و چندشکلیهای UCP میتوانند به عنوان نشانگرهای DNA برای انتخاب در فرآیند اصلاح نژاد مرغ بومی فارس مورد استفاده قرار گیرند.
Abadi M.M., Askari N., Baghizadeh A. and Esmailizadeh A. (2009). A directed search around caprine candidate loci provided evidence for microsatellites linkage to growth and cashmere yield in Rayini goats. Small Rumin. Res. 81, 146-151.
Adams S. (2002). Uncoupling protein homologs: emerging views of physiological function. Nutrition. 130, 711-714.
Argyropoulos G. and Harper M.E. (2002). Uncoupling proteins and thermoregulation. J. Appl. Physiol. 92, 2187-2198.
Askari N., Mohammadabadi M.R. and Baghizadeh A. (2011). ISSR markers for assessing DNA polymorphism and genetic characterization of cattle, goat and sheep populations. Iranian J. Biotechnol. 9, 222-229.
Boss O., Hagen T. and Lowell B.B. (2000). Uncoupling proteins 2 and 3, potential regulators of mitochondrial energy metabolism. Diabetes. 49, 143-156.
Chaudhuri L., Srivastava R.K., Kos F. and Shrikant P.A. (2016). Uncoupling protein 2 regulates metabolic reprogramming and fate of antigen‑stimulated CD8+ T cells. Cancer Immunol. Immunother. 65, 869-874.
Contreras L., Rial E., Cerdan S. and Satrustegui J. (2016). Uncoupling protein 2 (UCP2) function in the brain as revealed by the cerebral metabolism of (1–13C)-glucose. Neurochem. Res. 1, 1-7.
Dulloo A.G. and Samec S. (2001). Uncoupling proteins: their roles in adaptive thermogenesis and substrate metabolism reconsidered. British J. Nutr. 86, 123-139.
Evock-Clover C.M., Poch S.M., Richards M.P., Ashwell C.M. and McMurtry J.P. (2002). Expression of an uncoupling protein gene homolog in chickens. Comp. Biochem. Physiol. A: Mol. Integr. Physiol. 133, 345-358.
Fleury C., Neverova M. and Collins S. (1997). Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nat. Genet. 15, 269-272.
Gilmour A.R., Gogel B.J., Cullis B.R. and Thompson R. (2006). ASReml User Guide. Release 2.0 VSN International Ltd., Hemel Hempstead, HP1 1ES, United Kingdom.
Gimeno R.E., Dembski M. and Gimeno C.J. (1997). Cloning and characterization of an uncoupling protein homolog: a potential molecular mediator of human thermogenesis. Diabetes. 46, 900-906.
Guo J.J., Liu Y.J., Li M.X., Yang Y.J., Recker R.R. and Deng H.W. (2005). Linkage exclusion analysis of two candidate regions on chromosomes 7 and 11: leptin and UCP2/UCP3 are not QTLs for obesity in US Caucasians. Biochem. Biophys. Res. Commun. 332, 602-608.
Han R.H. (2008). Study on the polymorphisms in UCP3, IGF2, CAPN1 gene and its relationship with carcass and meat quality traits in Qinchuan cattle and its hybrid cattle. Ph D. Thesis. Northwest A and F Univ., Xianyang, China.
Himms-Hagen J. (1990). Brown adipose tissue thermogenesis: interdisciplinary studies. Fatig. Fract. Eng. Mater. Struct. 4, 2890-2898.
Himms-Hagen J. and Harper M.E. (2001). Physiological role of UCP3 may be export of fatty acids from mitochondria when fatty acid oxidation predominates: a hypothesis. Proc. Soc. Exp. Biol. Med. 226, 78-84.
Hirasaka K., Mills E.M., Haruna M., Bando A., Ikeda Ch., Abe T., Kohno Sh., Nowinski S.M., Lago C.U., Akagi K., Tochio H., Ohno A., Teshima-Kondo S., Okumura Y. and Nikawa T. (2016). UCP3 is associated with Hax-1 in mitochondria in the presence of calcium ion. Biochem. Biophys. Res. Commun. 472, 108-113.
Krauss S., Zhang C.Y. and Lowell B.B. (2002). A significant portion of mitochondrial proton leak in intact thymocytes depends on expression of UCP2. Proc. Natl Acad. Sci. America. 99, 118-122.
Li Q.L. (2006). Study on the relationship between polymorphism of UCP3 gene and biochemical index in serum of bovine. Ph D. Thesis. Northwest A and F Univ., Xianyang, China.
Liu Y.J., Liu P.Y., Long J., Lu Y., Elze L., Recker R.R. and Deng H.W. (2005). Linkage and association analyses of the UCP 3 gene with obesity phenotypes in Caucasian families. Physiol. Genom. 22, 197-203.
Moazeni S.M., Mohammadabadi M.R., Sadeghi M., Moradi Shahrbabak H., Esmailizadeh A.K. and Bordbar F. (2016). Association between UCP gene polymorphisms and growth, breeding value of growth and reproductive traits in Mazandaran indigenous chicken. Open J. Anim. Sci. 6, 1-8.
Mohammadabadi M.R., Nikbakhti M., Mirzaee H.R., Shandi M.A., Saghi D.A., Romanov M.N. and Moiseyeva I.G. (2010). Genetic variability in three native Iranian chicken populations of the Khorasan province based on microsatellite markers. Russian J. Genet. 46, 572-576.
Mohammadifar A., Faghih Imani S.A., Mohammadabadi M.R. and Soflaei M. (2013). The effect of TGFb3 gene on phenotypic and breeding values of body weight traits in Fars native fowls. J. Agric. Biotechnol. 5, 125-136.
Motloch L.J., Gebing T., Reda S., Schwaiger A., Wolny M. and Hoppe U.C. (2016). UCP3 Regulates single-channel activity of the cardiac mCa1. J. Membr. Biol. 249, 577-584.
Raimbault S., Dridi S., Denjean F., Lachuer J., Couplan E., Bouillaud F., Bordas A., Duchamp C., Taouis M. and Ricquier D. (2001). An uncoulping protein homologue putatively involved in facultative muscle thermogenesis in birds. Biochem. J. 353, 441-444.
Ricquier D. and Bouillaud F. (1997). The mitochondrial uncoupling protein: structural and genetic studies. Proc. Natl Acad. Sci. America. 56, 83-108.
Rudofsky G.J., Schroedter A., Schlotterer A., Voron’ko O.E., Schlimme M., Tafel J., Isermann B.H., Humpert P.M., Morcos M., Bierhaus A., Nawroth P.P. and Hamann A. (2006). Functional polymorphisms of UCP2 and UCP3 are associated with a reduced prevalence of diabetic neuropathy in patients with type 1 diabetes. Diabetes Care. 29, 89-94.
Saltzman E. and Roberts S.B. (1995). The role of energy expenditure in energy regulation: findings from a decade of research. Nutr. Rev. 53, 209-220.
SAS Institute. (2002). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Sherman E.L., Nkrumah J.D., Murdoch B.M., Li C., Wang Z., Fu A. and Moore S.S. (2008). Polymorphisms and haplotypes in the bovine neuropeptide Y, growth hormone receptor, ghrelin, insulin-like growth factor 2, and uncoupling proteins 2 and 3 genes and their associations with measures of growth, performance, feed efficiency, and carcass merit in beef cattle. J. Anim. Sci. 86, 1-16.
Shojaei M., Mohammad Abadi M.R., Asadi Fozi M., Dayani O., Khezri A. and Akhondi M. (2011). Association of growth trait and Leptin gene polymorphism in Kermani sheep. J. Cell. Mol. Res. 2, 67-73.
Taylor B.A. and Phillips S.J. (1996). Detection of obesity QTLs on mouse chromosome-1 and chromosome-7 by selective DNA pooling. Genomics. 34, 389-398.
Toubro S., Sorensen T.I.A., Ronn B. and Astrup A. (1996). Twenty four-hour energy expenditure: the role of body composition, thyroid status, sympathetic activity, and family membership. J. Clin. Endocrinol. Metabol. 81, 2670-2674.
Vignal A., Milan D., San Cristobal M. and Eggen A. (2002). A review on SNP and other types of molecular markers and their use in animal genetics. Genet. Sel. Evol. 34, 275-305.
Wang Q., Wang J., Hu M., Yang Y., Guo L., Xu J., Lei C., Jiao Y. and Xu J.C. (2016). Uncoupling protein 2 increases susceptibility to lipopolysaccharide-induced acute lung injury in mice. Mediat. Inflamm. 2016, 9154230.
York B., Truett A.A. and Monteiro M.P. (1999). Gene-environment interaction: a significant diet-dependent obesity locus demonstrated in a congenic segment on mouse chromosome 7. Mamm. Genom. 10, 457-462.
Yu G., Wang J., Xu K. and Dong J. (2016). Dynamic regulation of uncoupling protein 2 expression by micro RNA-214 in hepatocellular carcinoma. Biosci. Rep. 36, 1-6.
Zamani P., Akhondi M. and Mohammadabadi M.R. (2015). Associations of inter-simple sequence repeat loci with predicted breeding values of body weight in Sheep. Small Rumin. Res. 132, 123-127.
Zamani P., Akhondi M., Mohammadabadi M.R., Saki A.S., Ershadi A., Banabazi M.H. and Abdolmohammadi A.R. (2013). Genetic variation of Mehraban sheep using two intersimple sequence repeat (ISSR) markers. African J. Biotechnol. 10, 1812-1817.
