هضم‌پذیری آزمایشگاهی فیبر، تولید گاز و فعالیت آنزیمی باکتری‌های سلولولیتیک شترهای عربی تغذیه شده با علوفه زراعی و مرتعی
Subject Areas : Camel
پ. دادور
1
(Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran)
ط. محمدآبادی
2
(Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran)
م. ساری
3
(Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran)
ج. فیاضی
4
(Department of Animal Science, Faculty of Animal and Food Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran)
Keywords: آتریپلکس, شتر عربی, اندوگلوکاناز, اگزوگلوکاناز, هضم‌پذیری آزمایشگاهی,
Abstract :
این آزمایش برای ارزیابی فعالیت باکتری‌های سلولولیتیک شکمبه شترهای عربی تک کوهانه تغذیه شده با علوفه زراعی و مرتعی انجام شد. شترهای عربی فیستولا گذاری شده، برای 35 روز در دو دوره در قالب یک طرح چرخشی با علوفه زراعی و مرتعی تغذیه شدند (4 شتر برای هر علوفه). مایع شکمبه بعد از خوراکدهی صبحگاهی جمع‌آوری شد. مایع تلقیح باکتری‌های سلولولیتیک تهیه و تولید گاز، هضم‌پذیری و فعالیت آنزیمی اندازه‌گیری شد (6 تکرار). تیمارها شامل مایع شکمبه شترهای تغذیه شده با علوفه زراعیو کاه گندم یا آتریپلکس به عنوان سوبسترا و مایع شکمبه شترهای تغذیه شده با علوفه مرتعیو کاه گندم یا آتریپلکس به عنوان سوبسترا بودند. نتایج نشان داد بعد از 96 ساعت انکوباسیون، گاز تولید شده و ماده آلی واقعا هضم شده برای تیمارهای 1 و 4 بالاتر بود (01/0P≤). در حالیکه ازت آمونیاکی بعد از 24 و 48 ساعت، تحت تأثیر تیمارها قرار گرفت (05/0P≤). هضم‌پذیری ماده خشک، ماده آلی و الیاف نامحلول در شوینده خنثی باکتری‌ها توسط تیمارها تحت تأثیر قرار گرفت (01/0P≤). نتایج مشخص کرد فعالیت اندوگلوکاناز و اگزوگلوکاناز در تیمارهای 1 و 3 بعد از 48 ساعت انکوباسیون (01/0P≤) و مقادیر تیمار 4 بعد از 96 ساعت انکوباسیون بالاترین مقدار بود (01/0P≤). بنابراین علوفه مرتعی مانند آتریپلکس باعث تأخیر در شروع فعالیت تخمیر باکتری‌های شکمبه شترها شدند، بعداً تأخیر اولیه با تطابق یافتن به سوبسترا جبران می‌شود. همچنین تغذیه علوفه مرتعی C4به شترها، هضم‌پذیری فیبر و فعالیت آنزیمی باکتری‌های شکمبه را در مقایسه با علوفه زراعی C3بهبود داد.
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