تأثیر بنتونیت سدیم بر فراسنجه‌های خونی، قابلیت هضم خوراک و فراسنجه‌های تخمیری شکمبه گوسفندان نر بلوچی تغذیه شده با جیره آلوده به دیازینون

Subject Areas : Camel

م.ه.  اعظمی ¹ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
ع.م.  طهماسبی ² (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
و.  فروهر ³ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
ع.ع.  ناصریان ⁴ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)

Received: 2016-12-09 Accepted : 2017-04-15 Published : 2017-09-01

Keywords: دیازینون, فراسنجه‌های خونی, بنتونیت سدیم, گوسفندان نر بلوچی, قابلیت هضم مواد مغذی, فراسنجه‌های شکمبه,

Abstract :

به علت استفاده بیش از حد آفت‌کش­ها به منظور تأمین تقاضای بالای خوراک، بقایای آنها در خوراک دام­ها زیاد شده است. یافتن راهبردی جدید جهت کاهش آثار مضر آفت‌کش­ها کاملاً ضروری به نظر می­رسد. بنابراین وارسی آثار بنتونیت سدیم بر فراسنجه­های خونی، قابلیت هضم خوراک و فراسنجه­های تخمیری شکمبه در گوسفندان تغذیه شده با خوراک آلوده به دیازینون (آفت‌کش ارگانوفسفره) هدف این مطالعه بود. 8 گوسفند نر بلوچی (2±40 کیلوگرم) به یک طرح آزمایشی فاکتوریل 2 × 2 با چهار دوره 21 روزه، اختصاص داده شدند. تیمارها شامل: جیره شاهد، جیره شاهد به همراه 4 درصد بنتونیت سدیم، جیره شاهد به همراه 21 ppm آفت‌کش دیازینون و جیره شاهد به همراه 4 درصد بنتونیت سدیم و 21 ppm آفت‌کش دیازینون بودند. قابلیت هضم ماده خشک، پروتئین خام، NDF و ADF تحت تأثیر تیمارها قرار نگرفت (05/0‌>‌P). بنتونیت سدیم نوسانات اسیدیته شکمبه را پس از مصرف خوراک کاهش داد. سطح نیتروژن آمونیاکی شکمبه تیمارهای مختلف قبل از خوراک‌دهی مشابه بود اما 3 و 6 ساعت پس از مصرف خوراک سطح نیتروژن آمونیاکی به طور معنی‌داری در گروهی که خوراک آلوده به دیازینون مصرف کردند بیشتر بود (05/0‌>‌P). هموگلوبین، شمار سلول­های سفید و هماتوکریت تحت تأثیر تیمارهای آزمایشی قرار نگرفتند اما شمار گلبول­های قرمز و فعالیت آنزیم استیل کولین استراز به طور معنی‌داری در جیره­های حاوی آفت‌کش کاسته شد (05/0‌>‌P). نتایج مطالعه حاضر نشان داد که استفاده از سدیم بنتونیت به عنوان یک جاذب برای کاهش اثرات منفی دیازینون بر نوسانات اسیدیته و فعالیت آنزیم استیل کولین استراز مؤثر است. علاوه بر این اثری بر هموگلوبین، شمار گلبول­های سفید، هماتوکریت و قابلیت هضم مواد مغذی خوراک ندارد. بنابراین بنتونیت سدیم می­تواند به عنوان یک جاذب مؤثر دیازینون در جیره گوسفندان استفاده شود.

References:

Aazami M.H., Tahmasbi A.M., Ghaffari M., Naserian A.A., Valizadeh R. and Ghaffari A. (2013). Effects of saponins on rumen fermentation, nutrients digestibility, performance, and plasma metabolites in sheep and goat kids. Annu. Rev. Res. Biol. 3(4), 596-607.

Aghashahi A.R., Mirhadi S.A. and Moradishahre Babak M. (2005). Effects of natural bentonite (Montmorillonite), processed bentonite and clinopetilolite-rich tuff on the fermentation parameters, rumen microbial population and feedlot performance in male calves. Irnian J. Agric. Sci. 36, 3-10.

Aguilera-Soto J., Ramirez R., Arechiga C., Mendez-Llorente F., Lopez-Carlos M., Pina-Flores J., Medina-Flores C., Rodriguez-Frausto H., Rodriguez-Tenorio D. and Gutierrez-Banuelos H. (2009). Effect of feed additives on digestibility and milk yield of Holstein cows fed wet brewer grains. J. Appl. Anim. Res. 36(2), 227-230.

AOAC. (2002). Official Methods of Analysis. Vol. I. 18^th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.

Ashraf M.A., Maah M.J., Yusoff I. and Mehmood K. (2010). Effects of polluted water irrigation on environment and health of people in Jamber, District Kasur, Pakistan. Int. J. Basic Appl. Sci. 10(3), 37-57.

Betrosian A., Balla M., Kafiri G., Kofinas G., Makri R. and Kakouri A. (1995). Multiple systems organ failure from organophosphate poisoning. J. Clin. Toxicol. 33(3), 257-260.

Boucard T.K., Parry J., Jones K. and Semple K.T. (2004). Effects of organophosphate and synthetic pyrethroid sheep dip formulations on protozoan survival and bacterial survival and growth. FEMS Microbiol. Ecol. 47, 121-127.

Chamber J.E. and Levi P.A. (1992). Organophosphates: chemistry, fate and effects. Academic Press, San Diego, California.

Chegeni A., Li Y., Deng K., Jiang C. and Diao Q. (2013). Effect of dietary polymer-coated urea and sodium bentonite on digestibility, rumen fermentation, and microbial protein yield in sheep fed high levels of corn stalk. Livest. Sci. 157(1), 141-150.

Chen H., He X., Rong X., Chen W., Cai P., Liang W., Li S. and Huang Q. (2009). Adsorption and biodegradation of carbaryl on montmorillonite, kaolinite and goethite. Appl. Clay Sci. 46, 102-108.

Daneshvar N., Aber S., Khani A. and Rasoulifard M.H. (2007). Investigation of adsorption kinetics and isotherms of imidacloprid as a pollutant from aqueous solution by adsorption onto industrial granular activated carbon. J. Food Agric. Environ. 5, 425-429.

Debski B., Kania B.F. and Kuryl T. (2007). Transformations of diazinon, an organophosphate compound in the environment and poisoning by this compound. Ekológia. 26(1), 68.

Dixon J.B. and Schulze D.G. (2002). Soil Mineralogy with Environmental Applications: Soil Science Societyof America, Inc. Madison, Wisconsin, USA.

Donia M.A., Abou-Arab A., Enb A., El-Senaity M. and Abd-Rabou N. (2010). Chemical composition of raw milk and the accumulation of pesticide residues in milk products. Glob. Vet. 4(1), 06-14.

Eisenhour D.D. and Brown R.K. (2009). Bentonite and its impact on modern life. Elements. 5(2), 83-88.

Fisher L. and Mackay V. (1983). The effect of sodium bicarbonate, sodium bicarbonate plus magnesium oxide or bentonite on the intake of corn silage by lactating cows. J. Anim. Sci. 63(1), 141-148.

Gaikwad A.S., Karunamoorthy P., Kondhalkar S.J., Ambikapathy M. and Beerappa R. (2015). Assessment of hematological, biochemical effects and genotoxicity among pesticide sprayers in grape garden. Int. J. Occup. Med. Toxicol. 10, 1-11.

Galyean M. and Chabot R. (1981). Effects of sodium bentonite, buffer salts, cement kiln dust and clinoptilolite on rumen characteristics of beef steers fed a high roughage diet. J. Anim. Sci. 52, 1197-1204.

Garillo E., Pradhan R. and Tobioka H. (1995). Effects of activated carbon on growth, ruminal characteristics, blood profiles and feed digestibility in sheep. J. Anim. Sci. 8(1), 43-50.

Gaworecki K.M. and Klaine S.J. (2008). Behavioral and biochemical responses of hybrid striped bass during and after fluoxetine exposure. Aquat Toxicol. 88, 207-213.

Ha J., Emerick R. and Embry L. (1983). In vitro effect of pH variations on rumen fermentation, and in vivo effects of buffers in lambs before and after adaptation to high concentrate diets. J. anim. Sci. 56(3), 698-706.

Helal F. and Abdel-Rahman K. (2010). Productive performance of lactating ewes fed diets supplementing with dry yeast and / or bentonite as feed additives. J. Agric. Sci. 6(5), 489-498.

Hershberger T., Wilson L., Chase L., Rugh M. and Varela-Alvarez H. (1971). Effect of activated carbon on lamb performance and rumen parameters1. J. Dairy Sci. 54(5), 693-695.

Hofman J., Beerendonk E., Folmer H. and Kruithof J. (1997). Removal of pesticides and other micropollutants with cellulose-acetate, polyamide and ultra-low pressure reverse osmosis membranes. Desalinat. 113, 209-214.

Hua W., Bennett E.R. and Letcher R.J. (2006). Ozone treatment and the depletion of detectable pharmaceuticals and atrazine herbicide in drinking water sourced from the upper Detroit River, Ontario, Canada. Water Res. 40, 2259-2266.

Ivan M., Neill L., Alimon R. and Jalaludin S. (2001). Effects of bentonite on rumen fermentation and duodenal flow of dietary components in sheep fed palm kernel cake by-product. J. Anim. Feed Sci. Technol. 92(1), 127-135.

Jadhav K.B. and Rajini P.S. (2009). Evaluation of sublethal effects of dichlorvos upon Caenorhabditis elegans based on a set of end points of toxicity. J. Biochem. Mol. Toxic. 23, 9-17.

Jemec A., Drobne D., Tišler T., Trebše P., Roš M. and Sepčić K. (2007). The applicability of acetylcholinesterase and glutathione S-transferase in Daphnia magna toxicity test. Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol. 144, 303-309.

Judge S.J., Savy C.Y., Campbell M., Dodds R., Gomes L.K., Laws G., Watson A., Blain P.G., Morris C.M. and Gartside S.E. (2016). Mechanism for the acute effects of organophosphate pesticides on the adult 5-HT system. Chem. Biol. Interact. 245, 82-89.

Kazemi M., Tahmasbi A.M., Valizadeh R. and Naserian A.A. (2012). Toxic influence of diazinon as an organophosphate pesticide on parameters of dry matter degradability according to in situ technique. Int. J. Basic. Appl. Sci. 12(06), 229-233.

Khan O. (2001). Organophosphate poisoning in a group of replacement heifers and dry cows. Canadian Vet. J. 42(7), 561-567.

Kovac G., Reichel P., Seidel H. and Mudron P. (1998). Effects of sorbents during organophosphate intoxication in sheep. Czech J. Anim. Sci. 43, 3-7.

Lotti M. (1995). Cholinesterase inhibition: complexities in interpretation. Clin. Chem. 41(12), 1814-1818.

Malato S., Blanco J., Richter C., Milow B. and Maldonado M. (1999). Solar photocatalytic mineralization of commercial pesticides: methamidophos. Chemosphere. 38, 1145-1156.

McCullough M. (1974). Improving urea utilization in ruminants. Georgia Agric. Res. 15, 3-10.

Ministry of Agriculture, MOA, PRC (2004). Feeding Standard of Meatproducing Sheep and Goats (NY/T 816-2004). China Agricultural Press, Beijing, China.

Moretto A. and Johnson M. (1987). Toxicology of organophosphates and carbamates. Pp. 33-48. in Toxicology of Pesticides. L.G. Costa, C.L. Galli and S.D. Murphy, Eds, Springer, Berlin, Heidelberg.

Nistiar F., Hrusovský J., Mojzis J. and Mizik P. (1984). Distribution of dichlorvos in the rat and the effect of clinoptilolite on poisoning. Vet. Meal. 29, 689-698.

Nistiar F., Mojžiš J., Kovac G., Seidel H. and Racz O. (2000). Influence of intoxication with organophosphates on rumen bacteria and rumen protozoa and protective effect of clinoptilolite-rich zeolite on bacterial and protozoan concentration in rumen. Folia Microbiol. 6, 567-571.

Prasad K. and Chhabra A. (2001). Organo-chlorine pesticide residues in animal feeds and fodders. Indian J. Anim. Sci. 71(12), 1178-1180.

Raikwar M. and Nag S. (2003). Organochlorine pesticide residues in animal feeds. Pp. 54-57 in Proc. 40^th Annu. Conven. Chem.Indian Chemical Society, India.

Rumsey T., Williams E. and Evans A. (1975). Tissue residues, performance and ruminal and blood characteristics of steers fed ronnel and activated carbon. J. Anim. Sci. 40, 743-749.

Saleh M. (1994). Using of feed additives for feeding farm animals. Ph D. Thesis. Tanta University, Tanta, Egypt.

SAS Institute. (1999). SAS^®/STAT Software, Release 8. SAS Institute, Inc., Cary, NC. USA.

Schwartz C.C., Nagy J. and Streeter C. (1973). Pesticide effect on rumen microbial function. J. Anim. Sci. 37(3), 821-826.

Steel R.G. and Torrie J.H. (1960). Principles and procedures of statistics. McGraw-Hill Book Company, London, New York.

Stephenson R., Huff J., Krebs G. and Howitt C. (1992). Effect of molasses, sodium bentonite and zeolite on urea toxicity. Australian J. Agric. Res. 43, 301-310.

Van Cong N., Phuong N.T. and Bayley M. (2009). Effects of repeated exposure of diazinon on cholinesterase activity and growth in snakehead fish (Channa striata). Ecotoxicol. Environ. Saf. 72(3), 699-703.

Van Soest P.V., Robertson J. and Lewis B. (1991). Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583-3597.

Wallace R. and Newbold C. (1991). Effects of bentonite on fermentation in the rumen simulation technique (Rusitec) and on rumen ciliate protozoa. J. Agric. Sci. Camb. 116, 163-168.

Williams A.G. Withers S.E. (1993). Changes in the rumen microbial population and its activities during the refaunation period after the reintroduction of ciliate protozoa into the rumen of defaunated sheep. Canadian J. Microbiol. 31, 61-69.

Zertal A., Jacquet M., Lavédrine B. and Sehili T. (2005). Photodegradation of chlorinated pesticides dispersed on sand. Chemosphere. 58, 1431-1437.