استفاده از روش برون‌تنی تولید گازشکمبه‌ای برای ارزیابی ارزش غذایی پنج نسبت علوفه به کنسانتره

Subject Areas : Camel

س. اربابی ¹ , ت. قورچی ² , س. رمضانپور ³

1 - Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
2 - Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
3 - Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

Received: 2016-07-12 Accepted : 2017-01-31 Published : 2017-06-01

Keywords: باقلا, متان, تانن, تولید گاز برون‌تنی,

Abstract :

این پژوهش به منظور بررسی ترکیبات شیمیایی و تخمیر برون‌تنی پنج جیره با نسبت­های مختلف علوفه (یونجه) به کنسانتره (باقلا)، علوفه(صفر): کنسانتره (100)، علوفه 25): کنسانتره (75)، علوفه (50): کنسانتره (50)، علوفه (75): کنسانتره (25)، علوفه (100): کنسانتره (صفر) براساس ماده ­خشک انجام شد. نتایج نشان داد که ترکیبات شیمیایی مانند ماده­ آلی، چربی ­خام، پروتئین­ خام، دیواره­ سلولی، دیواره ­سلولی فاقد همی­سلولز، پروتئین نامحلول در شوینده خنثی، پروتئین نامحلول در شوینده اسیدی، فنل کل، تانن و تانن متراکم در پنج جیره متفاوت بودند (05/0>P). پس از اولین انکوباسیون جیره­های آزمایشی به مدت 96 ساعت، نیمه عمر تولید گاز محاسبه و دومین و سومین انکوباسیون در زمان t1/4 و 24 ساعت متوقف گردید. قابلیت هضم حقیقی برون­تنی ماده­ خشک و قابلیت هضم حقیقی ماده­ آلی با افزایش نسبت کنسانتره افزایش یافت (01/0>P). پروتین میکروبی (گرم/کیلوگرم ماده­ خشک) و انرژی قابل متابولیسم (مگاژول/کیلوگرم ماده­ خشک) در جیره‌هایی که با تجزیه­پذیری بالاتر، بیشتر بود. راندمان تولید پروتئین میکروبی (PF) (میلی‌گرم/میلی‌لیتر) در جیره­های آزمایشی مختلف در هر زمان t1/4 و 24 ساعت تفاوت معنی­داری نداشت و تنها جیره حاوی 100 درصد علوفه در زمان t1/4 دارای PF پا­یینتری (17/1 میلی­گرم در میلی­لیتر) بود. تولید گاز از بخش دارای پتانسیل تخمیر(B) و نرخ تولید گاز (c) در جیره­های دارای مواد متراکم بیشتر، به طور معنی­داری بالاتر بود (01/0>P). افزایش نسبت کنسانتره جیره منجر به کاهش pH و نیتروژن آمونیاکی مایع شکمبه شد و غلظت اسیدهای چرب را متأثر ساخت (01/0>P). میزان رهایش گاز متان در جیره‌های آزمایشی از 13 تا 16/17 گرم/کیلوگرم ماده خشک متغیر بود و بیشترین مقدار مربوط به جیره حاوی 100 درصد علوفه بود.

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