چندشکلی‌های تک نوکلئوتیدی (SNPs) جدید در ناحیه‌های اینترون 2 و اگزون 3 ژن لپتین در گاوهای Sumba Ongole

Subject Areas : Camel

و.پ.ب.  پوترا ¹ (Research Center for Biotechnology, Indonesian Institute of Science, Jl. Raya Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia)
پ.پ.  آگونگ ² (Biotechnology, Indonesian Institute of Science, Jl. Raya Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia)

Received: 2019-04-21 Accepted : 2019-07-30 Published : 2020-06-01

Keywords: چندشکلی, ژن لپتین, SNPs, گاو Pasundan, مقدار محتوای اطلاعات چندشکلی,

Abstract :

ژن لپتین (LEP) گاوی به ­طور وسیعی به­ عنوان یک ژن کاندیدا برای انتخاب مولکولی برای بهبود صفات تولیدی گاوها استفاده شده است. این پژوهش برای تعیین چندشکلی‌های تک نوکلئوتیدی (SNPs) در ژن LEP گاوهای Sumba Ongole (SO, Bos indicus) با استفاده از روش توالی­ یابی انجام شد. در این پژوهش مجموعه‌ای از 31 حیوان برای تجزیه و تحلیل استفاده شدند. تحقیق نشان داد که مجموعه‌ای از 16 SNPs در ژن LEP شناسایی شدند. یک توالی 2025 جفت بازی از ژن LEP در این مطالعه بررسی شد و شامل اینترون 2 (1002 جفت باز) و اگزون 3 (1023 جفت باز) بود. مقدار محتوای اطلاعات چندشکلی (PIC) از 0.06 (پایین) تا 0.37 (متوسط) متغییر بود. مجموع 16 چندشکلی در ژن LEP گاوهای SO ارزش PIC متوسط داشتند (0.25<PIC<0.50) و شامل 12 چندشکلی در اینترون 2 و چهار چندشکلی در اگزون 3 می‌شدند. چندشکلی‌های با ارزش PIC متوسط در اینترون 2 (g.2325G/T؛ g.2423A/C؛ g.2448C/T؛ g.2456C/G؛ g.2466C/T؛ g.2778T/A؛ g.2857G/A) و اگزون 3 (g.3260T/C؛ g.3272T/C؛ g.3356C/T؛ g.3468G/A) شناسایی شدند. چندشکلی g.3468G/A به­ عنوان چندشکلی جدید در ژن LEP گاوهای SO معرفی شد که در دیگر نژادهای گاو گزارش نشده است. این چندشکلی باعث تغییر اسیدآمینه از گلایسین (GGG) به آرژنین (AGG) شد. دو نوع از جهش در ژن LEP گاوهای SO شناسایی شد و شامل ترانسورژن (44 درصد) و ترانزیشن (56 درصد) بود. نتیجه گرفته شد که ژن LEP در گاوهای SO چندشکلی نشان داد و دارای پتانسیل برای انتخاب مولکولی در برنامه اصلاح نژادی از طریق تحقیقات عمیق است.

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