آثار مکمل جیره‌ای اسیدهای ارگانیک القاء‌کننده ایمنی محافظتی بر علیه کوکسیدیوز

Subject Areas : Camel

آ.ی. عبدوالهی ¹ , ایکس.جی. یو ² , ی.کیو. فو ³ , م.و. وانگ ⁴ , ن.اس. کی ⁵ , م.اچ. گیزا ⁶ , اس. کالون ⁷ , و.د. پان ⁸ , ایکس.ل. شی ⁹ , ی. فانگ ¹⁰ , اس. ویو ¹¹ , و. هو ¹² , ل.پی. تان ¹³ , کا.ایکس. لی ¹⁴ , ز. وانگ ¹⁵ , جی.جی. زو ¹⁶ , د.ی. فنگ ¹⁷ , جی.کیو. لی ¹⁸

1 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China|Department of Animal Science, Kano University of Science and Technology Wudil, PMB3244, Kano, Nigeria
2 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
3 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
4 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
5 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
6 - College of Animal Science, South China Agricultural University, Guangzhou, China
7 - Department of Animal Science, Njala University, Freetown, Sierra Leone|College of Animal Science, South China Agricultural University, Guangzhou, China
8 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
9 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
10 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
11 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
12 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
13 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
14 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
15 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
16 - College of Animal Science, South China Agricultural University, Guangzhou, China
17 - College of Animal Science, South China Agricultural University, Guangzhou, China
18 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

Received: 2019-02-01 Accepted : 2019-05-15 Published : 2020-03-01

Keywords: ایمنی, اسید ارگانیک, کوکسیدیوزها, سایتوکاین‌ها, سلول T,

Abstract :

این مطالعه آثار سه محصول از اسیدهای ارگانیک جیره‌ای (Acidomix® AFG؛ Activate® AD و Lacplus) روی ایمنی محافظتی بر علیه کوکسیدیوزها را ارزیابی کرد. 280 جوجه 1 روزه گوشتی به ‌طور تصادفی درون 8 گروه شامل 5 تکرار (7 پرنده به ازای هر تکرار)، شامل 2 کنترل (منفی=N؛ مثبت=P) و 6 گروه مکمل اسید ارگانیک (1 درصد AFG، 1 درصد DA، 1 درصد Lacplus، 2 درصد AFG، 2 درصد DA و 2 درصد Lacplus) تقسیم شدند. به استثنای کنترل N (آلوده نشده) ، تمامی 7 گروه به ‌طور خوراکی با 105 × 8 از اووسیت‌های هاگ‌آور شده Eimeria tenella در روز 12 بعد از هچ به چالش کشیده شدند. نمونه‌های خون، طحال و روده کور در روز 22 و 35 بعد از هچ جمع‌آوری شدند. رشد وزنی بدن، خارج شدن اووسیت مدفوعی، درجه تخریب، نرخ مرگ و میر و pH سکوم بررسی شدند. تست ELISA برای تشخیص خوشه مولکول‌های سلول‌ T افتراقی (CD4+ و CD8+) استفاده شده و واکنش زنجیره‌ای پلیمراز رونوشت‌بردار معکوس (RT-PCR) برای تشخیص کموکاین‌های پیش التهابی (INFγ) و بیان سایتوکاین‌ها (IL-8، IL-15 و IL-17) بکار گرفته شدند. نتیجه نشان داد که مکمل‌سازی اسیدهای ارگانیک به ‌طور معنی‌داری رشد وزنی بدن را افزایش، ضریب تبدیل خوراک را بهبود، و درجات جراحات و شاخص اووسیت (0.05>P) در مقایسه با گروه کنترل P (آلوده شده غیر مکمل) را کاهش داد. نرخ مرگ و میر در گروه کنترل P بالاتر بود. pH روده کور معنی‌دار بود (0.05>P) و حداکثر در گروه کنترل P مشاهده شد. جوجه‌های تغذیه شده با 2 درصد DA و 1 درصد Lacplus درصد بالاتری از مولکول‌های سلول T CD4 داشتند، اما مولکول‌های سلول T CD8 کمتری داشتند، که ارائه کننده یک عملکرد محافظتی از این زیرمجموعه‌های سلول T در پاسخ ایمنی ذاتی در مقابل عفونت E. tenella است. کموکاین‌های روده کور و طحال و بیان mRNA سایتوکاین‌ها شامل INFγ، IL-8، IL-15 و IL-17 سطوح بالاتری از رونویسی در مقایسه با کنترل‌های N و P نشان دادند، که نشان می‌دهد محصولات اسید ارگانیک ممکن است با بهبود تولید آنها آثار محافظتی خود را اعمال کرده باشند. نتیجه گرفته شده که Activate® DA در سطوح 2 درصد و Laxplus در سطوح 1 درصد هم در روز 22 و هم در روز 35 بعد از هچ بهترین آثار ضدکوکسیدیال را نشان داده‌اند.

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