اثر سطوح مختلف زرده تخم‌ مرغ روی خصوصیات ذخیره‪سازی منی بز سیاه بنگال در رقیق‌کننده تریس-زرده تخم ‌مرغ-سیترات-فروکتوز-گلیسرول

Subject Areas : Camel

ال.  گویجن سینق ¹ (Department Veterinary Gynaecology, Directorate of Veterinary and A.H. Services, Manipur, Sanjenthong, India)
ک.  رای ² (Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Science, WBUAFS, 37, K.B. Sarani, Kolkata, 700037, West Bengal, India)
ب.  سرکار ³ (Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Science, WBUAFS, 37, K.B. Sarani, Kolkata, 700037, West Bengal, India)

Received: 2015-04-11 Accepted : 2015-06-15 Published : 2016-03-01

Keywords: بز, نگهداری در شرایط انجماد, گلیسرول, رقیق‌کننده,

Abstract :

این مطالعه برای ارزیابی خصوصیات فیزیکی و مرفولوژیکی منی تازه و منی پیش از انجماد و یخ گشایی شده بز سیاه بنگال انجام شد. نمونه‪ها در رقیق­کننده تریس-زرده تخم­ مرغ-سیترات-فروکتوز-گلیسرول دارای 4 غلظت متفاوت (5/2، 5، 5/7 و 10 درصد) از زرده تخم مرغ عمل­آوری شدند. انزال­ها (48 انزال) به صورت هفتگی و به مدت 8 هفته از 6 بز بالغ با استفاده از واژن مصنوعی جمع­آوری شد. یک کاهش عمومی در مقادیر فراسنجه­های پیش و پس از انجماد از قبیل حرکت پیش­رونده اسپرم (درصد)، اسپرم زنده (درصد)، یکپارچگی اکروزوم (درصد) و اسپرم مثبت (درصد) در آزمون HOS، ولی یک افزایش کلی در اسپرم­های غیر ­طبیعی در مقایسه با اسپرم تازه برای رقیق­کننده­های حاوی غلظت­های متفاوت زرده تخم­ مرغ مشاهده شد. بهترین مقادیر برای تمامی فراسنجه­های منی با استفاده از رقیق­کننده حاوی 5/2 درصد زرده تخم ­مرغ به دست آمد. از بین غلظت­های زرده تخم ­مرغ، غلظت 10 درصد بالاترین اسپرم غیر طبیعی را سبب شد، که در مقایسه با غلظت­های 5/2 و 5 درصد معنی­دار (05/0P<)، ولی در مقایسه با غلظت 5/7 درصد غیر معنی­دار بود (05/0P>). حرکت پیش­رونده اسپرم تنها فراسنجه­ای بود که به طور ­معنی­داری (05/0P<) بین غلظت­های متفاوت رقیق کننده (5/2، 5، 5/7 و 10 درصد) متفاوت بود. نتیجه­گیری اینکه منی بز سیاه بنگال می­تواند به طور مؤثری با استفاده از رقیق­کننده تریس-زرده تخم­ مرغ-فروکتوز-گلیسرول حاوی 5/2 درصد زرده تخم ­مرغ (v/v) در شرایط انجماد نگهداری شود.

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