اثر عصاره برگ Spondias mombin بر تومور ناشی از مواد شیمیایی

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

اولوفونکه آکانجی ¹ , مکدونالد آیدو ² , آیدوا اوموتویی ³

1 - گروه علوم گیاهی و بیوتکنولوژی، دانشگاه آدکانله آجاسین، آکونگبا-آکوکو، ایالت اوندو، نیجریه.
2 - گروه زیست شناسی گیاهی و بیوتکنولوژی، دانشکده علوم زیستی، دانشگاه بنین، شهر بنین، ایالت ادو، نیجریه.
3 - گروه بیوشیمی، دانشگاه افه بابالولا، آدو اکیتی، ایالت اکیتی، نیجریه.

Received: 2022-03-25 Accepted : 2022-06-29 Published : 2022-06-22

Keywords: cancer, Extract, Trichloroacetic Acid, Ethidium bromide, Anacardiaceae, Spondias mombin, Solid tumor volume,

Abstract :

Background & Aim: A tumor is merely a mass of tissue that does not perform any physiological function; it can be either benign or malignant. Cancer is being regarded as a malignant ailment emerging from unrestricted cell division occurring in the body to make mass of tissues. Spondias mombin L.  (Family- Anacardiaceae) grows in coastal areas and in the rain forest into a big tree of up to 15 – 22 m. This study investigated the efficacy of S. mombin aqueous and n-hexane extracts in chemically induced tumor-bearing animals.Experimental:  Twenty-eight (28) healthy rats were used and grouped into seven (7). Six groups were induced with trichloroacetic acid and ethidium bromide leaving out normal control group for 28 days prior to treatments. LBH589 (standard anticancer drug, 500 mg/kg) served as positive control. At the termination of the experiments, the animals were sacrificed; solid tumor volume was determined, blood and tissue samples were collected for hematology and histopathology respectively.Results: The results showed very significant reduction in the solid tumour volumes of S. mombin leaves extracts treated groups compared to positive and untreated control groups. The result of hematological parameters showed that the hemoglobin content, red blood cell and white blood cell counts of all treated groups were near to normal control group. Normal histological features were observed in control group animals, the negative groups showed the manifestations of cancer, all the treatment groups showed significant levels of repair almost similar to normal features in control group particularly in intestines and lungs of the experimental animals.Recommended applications/industries: The present data provided substantial evidence that S. mombin leaves suppressed tumor growth in experimental animals and suggested that the extracts are potential antitumorigenic agent inhibiting chemically induced lung and intestinal tumors.

References:

Abdel-Hamid, N. M., Fawzy1, M. A., El-Moselhy, M. A. 2011. Evaluation of Hepatoprotective and Anticancer Properties of Aqueous Olive Leaf Extract in Chemically Induced Hepatocellular Carcinoma in Rats. American Journal of Medicine and Medical Sciences, 1(1): 15-22.

Acharya, N. A., Deepak, M. and Shrivastava, A. 2008. Indigenous Herbal Medicines: Tribal Formulations and Traditional Herbal Practices. India, Aavishkar Publishers Distributor, pp.520.

Adedokun, M. O., Oladoye, A. O., Oluwalana, S. A. and Mendie, I. I. 2010. Socio-economic importance and utilization of Spondias mombin in Nigeria. Asian Pacific Journal of Tropical Medicine, 3 (3): 232 - 234.

Alexander, R. R. and Grifiths, J. M. 1993. Haemotocrit determination by the cyanomethaemoglobin method In: Biochemical Methods, 2^nd Ed, John Willey and Sons, Inc. Publications. New York, pp.186– 187.

Bicas, J., Molina, G., Dioniso, P. A., Barros, F. F., Wagner, R., Marostica, M. and Pastore, G. 2011. Volatile constituents of exotic fruits from Brazil. Food Research International, 44: 1843 – 1855.

Calle, E. E., Carmen Rodriguez, M. D., Kimberly Walker- Thurmond, B. A., Michael J. and Thun, M. D. 2003. Overweight, obesity, and mortality from cancer in a prospectively studied cohort of U.S. adults. New England Journal of Medicine, 348: 1625- 1638.

Casado, R., Landa, A., Calvo, J., García-Mina, J. M., Marston, A and Hostettmann, K. 2011. Anti-inflammatory, antioxidant and antifungal activity of Chuquiraga spinosa. Pharmaceutical Biology, 49: 620- 626.

Chukwuemeka, S., Nworu, P. A., Akah, Festus B.C., Okoye, D. K., Toukam, J. U. and Charles, O. E. (2011). The leaf extract of Spondias mombin L. displays an anti-inflammatory effect and suppresses inducible formation of tumor necrosis factor-α and nitric oxide (NO). Journal of Immunotoxicology, 8 (1): 10 - 16.

Dacie, J. V. and Lewis, S. M. 2002. Practical Haematology, 8^th ed., Longman Group Ltd. Hong Kong, pp. 240.

 

 

De Vita, V. T. Jr., Hellman, S. and Rosenburg, S. A. 1993. Cancer: Principle and practice of oncology, 4th ed., J. B. Lippincott company, Philadelphia. pp. 34.

Duvall, C. S. (2006). On the origin of the tree Spondias mombin in Africa. Journal of Historical Geography, 32: 249- 266.

Hoagland, H. C. 1982. Hematologic complications of cancer chemotherapy. Seminal in Oncology, 9: 95- 102.

Kakar, S., Kamath, P. S., Burgart, L. J. 2004. Sinusoidal dilatation and congestion in liver biopsy: is it always due to venous outflow impairment? Archives of Pathology and Laboratory Medicine, 128 (8): 901- 904.

Kleiner D. E. 2005. Noncirrhotic portal hypertension: pathology and nomenclature. Clinical Liver Disease, 5 (5): 123-126.

Mita, E., Hayashi, N. and Lio, S. 1994. Role of Fas ligand in apoptosis induced by hepatitis C virus infection. Biochemical and Biophysical Research Communications, 204: 468– 474.

Musa, Y. M., Haruna, A. K., Ilyas, M., Yaro, A. H., Ahmadu, A. A. and Usman, H. 2007. Phytochemical, analgesic and anti-inflammatory effects of the ethyl acetate extract of the leaves of Pseudocedrella kotschyii. African Journal of Traditional, Complementary and Alternative Medicine, 5 (1): 92– 96.

Nworu, C. S., Akah, P. A., Okoli, C. O., and Okoye, T. C. 2007. Oxytocic activities of leaf extract of Spondias mombin (Anacardiaceae). Pharmaceutical Biology, 45: 366– 371.

Oberling, C. and GueRin, M. 1954. The role of viruses in the production of cancer. Advancement in Cancer Research, 2: 353-423.

Ovchinnikov, D. A. 2008. Macrophages in the embryo and beyond: much more than just giant phagocytes. Genesis, 46 (9): 447– 462.

Price, V. E. 1958. Anemia in cancer. Advancement in Cancer Research, 5: 199- 200.

Rady, M. I. and Amany, M. O. 2016. Histological alterations in the liver of mother rats and its weanlings fed on fried bread and the protective effect of curcumin. Journal of the Egyptian Society of Parasitology, 35: 19- 25.

Rodrigues, B. and McNeill, J. H. 1992. The diabetic heart: metabolic causes for the development of cardiomyopathy. Cardiovascular Research, 26: 913- 922.

Ruby, A. J., Kuttan, G., Babu, K. D., Rajasekharan, K. N. and Kuttan, R. (1995). Antitumor and antioxidant activity of natural curcuminoids. Cancer Letter, 94: 79- 83.

Song, X., Jiabei, W., Tongsen, Z., Ruipeng, S., Yingjian, L. N., Bhatta, D. Y., Shangha, P., Jiaren, L., Hongchi, J. and Lianxin, L. 2013. LBH589 Inhibits proliferation and metastasis of hepatocellular carcinoma via inhibition of gankyrin/stat3/akt pathway. Molecular Cancer, 12: 114– 1168.

Tominoa, T., Yo-ichi, Y. T., Iguchia, S. I., Mizuki, N. T., Ikegamia, T. Y., Yuji, S. H., Kawanakaa, T. I., Shinichi, A. K. and Shirabea, Y. M. 2014. Spontaneous massive necrosis of hepatocellular carcinoma with narrowing and occlusion of the arteries and portal veins. Case Reports in Gastrointestinal, 8: 148– 155.