فهرس المقالات pluripotency

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  1 - Signaling Roadmap Modulating Chicken Primordial Germ Cells Proliferation and Self-Renewal
  M. Zare S.Z. Mirhoseini S.N. Hassani S. Ghovvati
  In the life science technologies, the chicken Primordial Germ Cells (PGCs) are recognized as the most practical cells compared to the other chicken stem cells. For this purpose, the isolation and long-term culture of these cells are of great importance for the productio أکثر

  In the life science technologies, the chicken Primordial Germ Cells (PGCs) are recognized as the most practical cells compared to the other chicken stem cells. For this purpose, the isolation and long-term culture of these cells are of great importance for the production of therapeutic proteins, such as monoclonal antibodies, vaccines, endangered species protection, and chimeric bird production. However, one of the major challenges of working with these cells is their lack of proliferation and self-renewal ability in the laboratory environment. Recent researches on chick PGCs have shown that the pluripotency-related signaling pathways active in these cells are highly effective in their in vitro proliferation and self-renewal. Therefore in this review, we tried to summarize and evaluate the mechanisms of the most important pluripotency-related signaling pathways in chicken PGCs, which may result in achieving the reproducible line for these cell types. Studies have shown that one method to induce pluripotency in PGCs is to manipulate signaling pathways, including TGF-β and Wnt/β-catenin, using growth factors and small molecules. Along with activation of signaling pathways involved in self-renewal, improving culture conditions can be an effective way to achieve chicken PGC cell line. It could be concluded that providing a defined culture condition and activating specific signaling pathways can lead to induction of proliferation in chick PGCs. تفاصيل المقالة
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  2 - Comparative Expression Analysis of Spermatogonial Stem Cell Markers in Cattle and Sheep
  F. Nasri Ahangar M. Zandi M.R. Sanjabi A. Ghaedrahmati
  Spermatogenesis is supported by stem cells called spermatogonial stem cells (SSCs), which are capable of transmitting information to the next generation. However, little is known on the specific markers of SSCs in farm animals. We investigated the expression of cdh1, cm أکثر

  Spermatogenesis is supported by stem cells called spermatogonial stem cells (SSCs), which are capable of transmitting information to the next generation. However, little is known on the specific markers of SSCs in farm animals. We investigated the expression of cdh1, cmyc, bcl6b, plzf, gfra1, nanog, vasa, thy1 and uchl1, as specific markers of SSCs, in bovine and ovine SSCs. The expression of the studied genes was conducted by real-time polymerase chain reaction method. For this reason, the enzymatic digestion process for two times was used to achieve SSCs from male calves and ram lambs testes. Then, filtration and differential plating techniques increased the SSC number in the cell suspension caused by mechanical and enzymatic digestions. Sertoli cells treated with Mitomycin-C were applied to obtain the feeder layer. Culturing of the stem cells was done on Sertoli cell feeder layer. Our results revealed that the expression of nanog and plzf was similar in bovine and ovine SSCs. Unlike bovine SSC colonies, cdh1 gene was not expressed in colony of ovine SSCs and its use as a specific marker of sheep SSCs is not suggested. The expression of uchl, vasa, thy1 and cdh1 genes was significantly higher in bovine SSCs and the bcl6b and cmyc expression was significantly higher in ovine SSCs compared to each other (P<0.05). From the results of this study nanog, plzf, uchl1, vasa, thy1 and cdh1 are suggested as markers of bovine and nanog, plzf, bcl6b and cmyc genes as markers of ovine SSCs. تفاصيل المقالة