فهرس المقالات genetic polymorphism

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  1 - Analysis of Growth Hormone Gene in Alpine and Saanen Goats Using PCR-SSCP Method
  ر. خاتمی نژاد س. یوسفی م. آهنی آذری
  In this study the genetic polymorphism of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH g أکثر

  In this study the genetic polymorphism of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH genewas amplified by polymerase chain reaction (PCR). PCR products were analyzed using single strand conformation polymorphism (SSCP) method on polyacrylamide gel. The results indicated that there are four (G1, G2, G3 and G4) conformational patterns with frequencies 0.38, 0.21, 0.23 and 0.18 for Alpine goat and 0.48, 0.21, 0.17 and 0.14 for Saanen goat, respectively. These results revealed that GH gene was polymorph in this studyand showed that PCR-SSCP is an appropriate tool for detecting polymorphism and evaluating genetic variability. تفاصيل المقالة
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  2 - Polymorphisms in GDF9 Gene and Its Relationship with Litter Size in Five Breeds of Black Goats
  G.Q. Zhu Q.I. Wang Y.G. Kang Y.Z. Lv B.Y. Cao
  In this study, we investigated the relationship between the genetic polymorphism of growth differentiation factor 9 (GDF9) genes and the litter size in 384 individuals of five breeds of black goats. Four pairs of primers were designed to detect single nucleotide polymor أکثر

  In this study, we investigated the relationship between the genetic polymorphism of growth differentiation factor 9 (GDF9) genes and the litter size in 384 individuals of five breeds of black goats. Four pairs of primers were designed to detect single nucleotide polymorphism of GDF9 gene in goats by PCR-SSCP. The least square was used to analyze the relation between different genotypes and the litter size. The results showed that the PCR products from primer pair 1 (P1) displayed polymorphisms in three genotypes (AA, AB and BB) in big foot (BF) and Jintang (JT) black goats. For primer pairs of P2, P3 and P4, there was no polymorphism. The sequencing results revealed that there was a single nucleotide mutation (A792→G) in exon 2 of GDF9 gene in BF and JT black goats, and this mutation resulted in an amino acid change: valine→isoleucine. In BF and JT black goats, the average litter size in the third parity was significantly higher in genotype AA than both genotypes of AB and BB while the average little size of genotype AB was higher than that of genotype BB in the same parity. GDF9 gene could be therefore considered as a candidate gene for marker-assisted selection of litter size trait in goats. تفاصيل المقالة
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  3 - Polymorphism of some Genes Associated with Meat-Related Traits in Egyptian Sheep Breeds
  کا.ف. ماهروس م.اس. حسنان م. ابدل مردی اچ.آی. شافی اچ.ای. روشدی
  The genetic polymorphism of some genes related to meat production in three Egyptian sheep breeds (Barki, Rahmani and Osseimi) was studied. The candidate genes were: Calpastatin, Myostatin, Diacylglycerol-acyltransferase1, Insulin-like growth factor binding protein-3 and أکثر

  The genetic polymorphism of some genes related to meat production in three Egyptian sheep breeds (Barki, Rahmani and Osseimi) was studied. The candidate genes were: Calpastatin, Myostatin, Diacylglycerol-acyltransferase1, Insulin-like growth factor binding protein-3 and Booroola fecundity gene. The technique applied was the restriction fragment length polymorphism for the polymerase chain reaction products. Polymorphism was found in the genes: Calpastatin, MyostatinandDiacylglycerol-acyltransferase 1, while no polymorphism was exhibited by the other two genes, Insulin-like growth factor binding protein-3 and the Booroola fecundity in the three breeds understudy. Calpastatin locus digested with MspI had two genotypes MM and MN. The highest allelic frequency was for allele M. The same locus Calpastatin digested with NcoI also exhibited two genotypes MM and MN. The NN genotype was absent with both the MspI and the NcoI enzymes in all breeds. Myostatin digested with DraI had two genotypes AB and BB. The AA genotype cannot be detected. The highest allelic frequency was for allele B. Diacylglycerol-acyltransferase1 digested with AluI showed two genotypes CC and CT. The highest allelic frequency was for allele C. The detected CT genotype might explain the moderate intramuscular fat content and muscle marbling in the Egyption sheep breeds. Each of the remaining two loci (Insulin-like growth factor binding protein-3 and the Booroola fecundity) had only one genotype, BB genotype for Insulin-like growth factor binding protein-3 digested with HaeIII and ++ genotype for the Booroola fecundity digested with AvaII enzyme, therefore they are not recommended in the selection program. The result of Chi-square analysis indicated that the three Egyptian sheep breeds were in Hardy-Weinberg equilibrium. تفاصيل المقالة