فهرس المقالات acrosome

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  1 - Effect of Tempol in Sasaki Diluent on the Quality and Fertility of Cryopreserved Chicken Semen
  M. Shanmugam R.K. Mahapatra
  Sperm are exposed to oxidative stress during cryopreservation leading to damage of membrane lipids. In the present study, the effects of adding Tempol during cryopreservation of chicken semen on post-thaw semen parameters and fertility were investigated. Adult PD-1lines أکثر

  Sperm are exposed to oxidative stress during cryopreservation leading to damage of membrane lipids. In the present study, the effects of adding Tempol during cryopreservation of chicken semen on post-thaw semen parameters and fertility were investigated. Adult PD-1linesemen were cryopreserved using 4% dimethyl sulfoxide (DMSO) in Sasaki diluent (SD). Tempol (1 and 5 mM) was added to the semen-cryopreservation mixture at final concentrations. The semen with additives was filled in 0.5 mL French straws and exposed to liquid nitrogen vapours for 30 min and then stored in liquid nitrogen. The semen straw was thawed at 5 ˚C for 100 seconds and evaluated for sperm motility, live, abnormal, and acrosome intact sperm. The seminal plasma was evaluated for lipid peroxidation. The fertilization potential of the cryopreserved sperm was evaluated after insemination in the PD-1line hens. Sperm parameters after thawing were significantly (P<0.05) lower in the cryopreserved groups compared to fresh semen. Lipid peroxidation was significantly (P<0.05) higher in cryopreserved groups. Fertility was significantly (P<0.05) lower in all the cryopreserved groups. In conclusion, the addition of template cryo diluent did not improve the post-thaw semen parameters or fertility. تفاصيل المقالة
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  2 - Insulin Like Growth Factor-1 Fortification Improves Post Thaw Sperm Quality and Fertility of Goat
  K. Sharma R. Ranjan S. Gupta
  Insulin like growth factor-1 (IGF-I) has an antioxidant effect to maintain sperm motility. The present study designed to evaluate the effect of different levels IGF-1 on semen characteristic after cryopreservation of buck semen. Fifty ejaculates were collected and were أکثر

  Insulin like growth factor-1 (IGF-I) has an antioxidant effect to maintain sperm motility. The present study designed to evaluate the effect of different levels IGF-1 on semen characteristic after cryopreservation of buck semen. Fifty ejaculates were collected and were extended with tris- citric acid- fructose diluent with 10% egg yolk and 6% glycerol with sperm concentrations of 4 × 108 mL−1. The effect of IGF-1 concentration showed significant differences (P<0.05) on most of studied traits (sperm motility, live sperm count, acrosomes integrity, hypo osmotic swelling positive spermatozoa, malondialdehyde (MDA)) and were significantly (P<0.05) highest in 250 ng/mL of IGF-1in post thaw semen. The post thaw progressive motility, live, hypo osmotic swelled and acrosome intact sperm increased by 25.26%, 26.77%, 30.35% and 25.52%, respectively in 250 ng/mL IGF1supplemented group in compare with control group. The 250 ng/mL IGF-1 supplemented group showed the lowest MDA content (P<0.05) in compare with other studied groups. The fortification 250 ng/mL IGF-1 reduced the MDA content by 31.16% more than the control group. The results suggested that IGF-1 protects sperm membrane lipid peroxidation by lowering MDA production by reducing the harmful effect of reactive oxygen species. Eight out of ten goats were conceived and kidded by using supplemented of 250 ng/mL IGF-1 and three goats were kidded by non-supplemented (control) frozen semen straw through artificial insemination (AI). The success of kidding was 40.00% in supplemented group (250 ng/mL IGF-1) than 30.00% in non-supplemented group (control) on actual kidding basis. تفاصيل المقالة